Duct-to-mucosa Technique

The duct-to-mucosa technique is a surgical method that directly connects the pancreatic duct to the mucosal lining of the jejunum, most often during pancreatic reconstruction after pancreaticoduodenectomy. It works by aligning the opened pancreatic duct with a small jejunal opening and securing both edges with fine sutures, allowing pancreatic secretions to drain into the digestive tract while limiting leakage into surrounding tissue. This pancreaticojejunostomy requires accurate duct identification, gentle tissue handling, and a tension-free anastomosis. In medicine, it restores pancreatic drainage and is studied for its potential to reduce postoperative pancreatic fistula and related complications.

Duct-to-mucosa Technique - Related Videos

Research

JoVE Journal - Medicine

Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct

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Cited by 17 •

2011

A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary glands.

Isolation of Lymphocytes from Mouse Genital Tract Mucosa

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Cited by 10 •

2012

An efficient way to isolate lymphocytes from mouse genital tract is described. This method takes advantage of enzyme digestion and Percoll gradient separation to allow efficient isolation. This technique is also adaptable to for use in other...

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants

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Cited by 16 •

2013

We introduce a novel method for the maintenance of human intestinal mucosa in culture and monitoring of the response to various types of stimuli over at least 24 hrs. With our method, the polarity of the tissue is maintained, allowing for a physiological stimulation via the apical route.

Esophageal 3D Raft Culture Model: An In Vitro Culture Technique to Generate Human Esophageal Mucosa Model

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2023

This video describes an in vitro culture technique to generate human organotypic esophageal constructs by co-culturing primary human esophageal fibroblast and squamous epithelial cells within a decellularized porcine scaffold. These 3D culture models mimic the function and physiology of their in vivo tissue counterparts.

Research

JoVE Journal - Biology
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Cryosectioning Method for Microdissection of Murine Colonic Mucosa

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Cited by 5 •

2015

Here we describe a simple method for the isolation of spatially distinct murine colonic epithelial surface cells from the underlying crypt-base cells.

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