Lung Tissue Imaging
Lung Tissue Imaging - Related Videos
02:42
Imaging Bacterial Localization and Host Interaction in Human Lung Tissue
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2026
Source: Mills, B., et al., Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy. J. Vis. Exp. (2017)This video demonstrates real-time imaging of fluorescently labeled Staphylococcus aureus in suspension and lung tissue using fibered confocal fluorescence microscopy.
05:55
Whole Lung Agarose Inflation: A Method of Preparing Lungs for Ex vivo Live Imaging
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2023
This video describes a method of preparing lungs for ex vivo live imaging to study interactions between cancer cells and stromal cells, using agarose for inflation. In the sample protocol, agarose inflation procedure is described before observing live cellular interactions.
11:19
Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment
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Cited by 6 •
2016
We describe a relatively simple method for ex vivo live imaging of the tumor cell-stroma interactions within lung metastasis, utilizing fluorescent reporters in mice. Using spinning-disk confocal microscopy, this technique enables visualization of live cells for at least 4 hr and could be adapted to study other inflammatory lung conditions.
11:26
Real-time X-ray Imaging of Lung Fluid Volumes in Neonatal Mouse Lung
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Cited by 1 •
2016
We present a protocol to assess the rate of alveolar fluid clearance or pulmonary edema in neonatal mouse lung using X-ray imaging technology.
07:19
Long-term High-Resolution Intravital Microscopy in the Lung with a Vacuum Stabilized Imaging Window
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Cited by 32 •
2016
This protocol describes the use of multiphoton microscopy to perform long-term high-resolution, single cell imaging of the intact lung in real time using a vacuum stabilized imaging window.
FAQs
Each modality emphasizes a different source of contrast. Differences in X-ray attenuation can show structural variation, while light scattering reflects how tissue affects illumination. Fluorescence from labeled cells or tissue components adds molecular specificity. Comparing these signal types helps investigators distinguish anatomy from cellular or molecular features within pulmonary samples.
Sectioning separates tissue into interpretable planes, making structures easier to examine at selected depths. Three-dimensional reconstruction then combines spatial information to represent relationships among alveoli, airways, and blood vessels. This added context can reveal tissue organization and remodeling patterns that may be difficult to understand from a single two-dimensional view.
The choice depends on whether the primary question concerns overall structure, optical tissue behavior, or labeled biological components. Attenuation-based imaging supports structural assessment, scattering-based methods provide information related to light interactions within tissue, and fluorescence can localize selected cells or components. Combining approaches may connect morphology with more specific biological findings.
Whole-organ views provide broad spatial context, whereas cellular-scale images resolve localized changes within pulmonary tissue. Examining multiple scales allows researchers to relate large patterns to microscopic features, such as how tissue remodeling or inflammation appears across an organ and within individual cells. This multiscale perspective supports more complete disease characterization.
A study first establishes whether the goal is structural, molecular, or multiscale analysis. Investigators then select an imaging approach based on its detectable signal and decide whether tissue sectioning, labeled components, or three-dimensional reconstruction is needed. The resulting images are interpreted in relation to pulmonary structures and the biological question being investigated.
The approach can be applied to inflammation, fibrosis, infection, tumors, and tissue remodeling. Imaging reveals how these processes affect pulmonary organization and, when labeled components are used, where selected cellular or tissue features occur. These observations help investigators compare disease patterns, examine underlying changes, and evaluate how pathology is distributed within the lung.
Microscopic findings can be related to clinical observations by showing how structural and molecular changes correspond to respiratory disease. This connection supports disease characterization and treatment assessment while also guiding development of improved diagnostic tools. In medicine, the value lies not only in producing images, but in linking tissue-level evidence with clinically relevant interpretation.