Transgenic Rabbit Models
Transgenic Rabbit Models - Related Videos
01:36
Anesthesia and Monitoring in a Rabbit Model
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Anesthesia is an essential component of rabbit surgical and experimental procedures because it prevents pain, reduces stress responses, and helps immobilize the animal during handling and manipulation when general anesthesia is...
02:24
Tracheal Cannulation in Rabbit Model: A Procedure to Insert Tracheal Cannula into the Rabbit Trachea
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2025
This video demonstrates the surgical creation of an opening in the rabbit trachea to insert a tracheal cannula for artificially ventilating the lungs.
10:59
Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
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Cited by 32 •
2013
Recent development in gene targeting tools makes production of knockout (KO) rabbits possible. In the present work, we generated five Apolipoprotein (Apo) C-III KO rabbits using Zinc Finger Nucleases (ZFN). This work demonstrated that ZFN is a highly efficient method to produce KO rabbits.
13:59
Assaying β-amyloid Toxicity using a Transgenic C. elegans Model
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Cited by 76 •
2010
The intensely studied nematode worm Caenorhabditis elegans can be transgenically engineered to express the human β-amyloid peptide (Aβ). Induced expression of Aβ in C. elegans muscle leads to a rapid, reproducible paralysis phenotype that can be used to monitor treatments that modulate Aβ toxicity.
13:58
A Rabbit Model of Durable Transgene Expression in Jugular Vein to Common Carotid Artery Interposition Grafts
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Cited by 2 •
2018
This method describes the placement of interposition vein grafts in rabbits, the transduction of the grafts, and the achievement of durable transgene expression. This allows the investigation of physiological and pathological roles of transgenes and their protein products in grafted veins, and testing of gene therapies for vein graft disease.
FAQs
The introduced DNA sequence can become integrated into the rabbit genome, allowing it to persist as cells divide and potentially pass to subsequent generations. Its biological effect depends on whether the sequence is introduced, altered, or regulated in a way that produces the intended gene activity. This relationship helps researchers connect a defined genetic change with observable disease-related traits.
Regulated or selected expression helps researchers examine gene activity in particular tissues rather than treating the animal as biologically uniform. That distinction is important when investigating how a molecular change contributes to disease in a specific organ or system. It can also make the resulting phenotype more informative for studying mechanisms in cardiovascular, metabolic, immune, ocular, or neurological disorders.
Researchers combine genotyping with phenotype analysis. Genotyping determines whether the intended sequence or alteration is present, while phenotype analysis examines the animal’s observable biological characteristics and disease-related changes. Comparing these findings allows investigators to relate the molecular modification to its effects and assess whether the model reproduces features relevant to human disease.
Their larger size and physiology can support procedures and longitudinal measurements that complement findings from mouse studies. This makes rabbits useful when investigators need to follow changes over time or perform measurements that benefit from a larger living system. They therefore provide an additional experimental context rather than simply replacing smaller animal models.
A typical workflow begins by preparing a DNA construct containing the intended genetic sequence or regulatory design. Researchers deliver it to embryos or reproductive cells, then identify resulting animals through genotyping. They subsequently analyze phenotype to determine how the genetic change is expressed and whether it produces characteristics relevant to the disease under investigation.
These models support investigations across cardiovascular, metabolic, immune, ocular, and neurological disorders. In each area, researchers can examine how altered gene activity relates to disease features in a living system. The range of applications makes the platform useful for studying both organ-specific mechanisms and broader connections between molecular changes and human disease.
Transgenic rabbit models can help connect molecular changes with disease mechanisms, biomarkers, and therapeutic evaluation. Genotype and phenotype findings show how the alteration affects the animal, while measurements collected over time can reveal disease-related progression. Together, these outcomes support interpretation of biological pathways and assessment of potential treatments in a living system.