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A Rabbit Model of Durable Transgene Expression in Jugular Vein to Common Carotid Artery Interposition Grafts
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A Rabbit Model of Durable Transgene Expression in Jugular Vein to Common Carotid Artery Interposition Grafts

A Rabbit Model of Durable Transgene Expression in Jugular Vein to Common Carotid Artery Interposition Grafts

DOI:
10.3791/57231-v

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13:58 min

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September 10, 2018

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, ,
1Department of Medicine,University of Washington School of Medicine

Chapters

  • 00:04Title
  • 00:37Vein Graft Surgery
  • 06:57Gene Transfer Surgery after Graft Placement
  • 11:09Vein Graft Harvest Surgery
  • 12:05Results: High-level β-galactosidase Expression in Jugular Vein to Common Carotid Artery Interposition Grafts
  • 13:09Conclusion

Summary

Automatic Translation

Automatic Translation

This method describes the placement of interposition vein grafts in rabbits, the transduction of the grafts, and the achievement of durable transgene expression. This allows the investigation of physiological and pathological roles of transgenes and their protein products in grafted veins, and testing of gene therapies for vein graft disease.

Tags

Rabbit ModelTransgene ExpressionVein GraftCommon Carotid ArteryGraft FailureVein Graft EndotheliumSurgical ProtocolDissectionLigationArteriotomyVein Grafting

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