Brain Reperfusion
Brain Reperfusion - Related Videos
03:40
TTC Staining of Rat Brain Tissue Slices: A Staining Procedure to Differentiate Viable and Necrotic Tissue in Tissue Slices After Ischemia-Reperfusion Injury
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2025
This video describes the 2,3,5-triphenyl-2H-tetrazolium chloride staining procedure to visualize rat brain tissue slices and differentiate viable and damaged tissue following middle cerebral artery occlusion and reperfusion.
08:56
Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction
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Cited by 46 •
2015
Ischemia-Reperfusion (IR) injury is associated with a high rate of morbidity and mortality. The goal of the in vitro model of oxygen-glucose deprivation and reoxygenation (OGD-R) described here is to assess the effects of ischemia reperfusion injury on a variety of cells, particularly in blood-brain barrier (BBB) endothelial cells.
07:07
Murine Model of Intestinal Ischemia-reperfusion Injury
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Cited by 52 •
2016
Here we describe the detailed procedure of intestinal ischemia-reperfusion in mice which results in reproducible injury without mortality to encourage the standardization of this technique across the field. This model of intestinal ischemia-reperfusion injury can be utilized to study the cellular and molecular mechanisms of injury and regeneration.
10:30
Surgical Approach for Middle Cerebral Artery Occlusion and Reperfusion Induced Stroke in Mice
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Cited by 16 •
2016
In order to understand the pathophysiology of stroke, it is important to use reliable models. This paper will describe one of the most frequently used stroke models in mice, termed the middle cerebral artery occlusion (MCAo) model (also termed the intraluminal filament or suture model) with reperfusion.
13:42
A Murine Closed-chest Model of Myocardial Ischemia and Reperfusion
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Cited by 19 •
2012
Surgical trauma induces an inflammatory response. Cytokines and endogenous ligands are known to modulate myocardial infarct size following ischemia and reperfusion. We present a modified closed-chest model of murine ischemia and reperfusion using hanging weights to minimize effects of thoracotomy.
FAQs
Restored flow can trigger a secondary injury response because oxygen re-enters tissue and may promote reactive oxygen species. Blood-brain barrier disruption, inflammation, and cerebral edema may also follow. These effects make reperfusion a biological event to monitor, not merely a successful reopening of circulation, when assessing how much neural tissue remains protected or injured.
Blood-brain barrier disruption is one of the tissue-level consequences associated with the return of flow. Along with inflammation and cerebral edema, it indicates that reperfusion may affect the neural environment beyond oxygen and nutrient delivery. Tracking these changes helps neuroscience studies characterize ischemia-reperfusion injury and evaluate whether an intervention preserves neuronal function.
Recanalization addresses a blocked vessel by restoring a route for blood flow, whereas recovery of circulation describes the return of perfusion without specifying that a particular blockage was reopened. Both can reintroduce oxygen and nutrients, but the distinction helps frame experimental or clinical assessment of how reperfusion was achieved.
Reperfusion imaging can provide evidence about restored blood flow and the extent of neural injury after ischemia. Its value extends beyond visualization: it supports treatment decisions, neuroprotection research, and development of therapies intended to preserve neuronal function. Imaging therefore connects the vascular event with assessment of tissue outcome and therapeutic relevance.
Experimental models provide a way to examine ischemia-reperfusion injury and evaluate potential interventions aimed at neuroprotection. By focusing on what happens when circulation returns, these models support investigation of both beneficial oxygen and nutrient reintroduction and harmful responses such as reactive oxygen species, inflammation, barrier disruption, and edema. Their results inform therapy development.
Brain reperfusion research is relevant to ischemic stroke, cardiac arrest, and related cerebrovascular conditions. In these settings, researchers can examine whether restored circulation is associated with limited neural injury or with reperfusion-related complications. The broader goal is to guide treatment decisions and develop neuroprotective approaches that preserve neuronal function after an ischemic interruption.