Oxygen Gradients
Oxygen Gradients - Related Videos
11:23
Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients
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Cited by 13 •
2017
The control of chemical and oxygen gradients is essential for cell cultures. This paper reports a polydimethylsiloxane-polycarbonate (PDMS-PC) microfluidic device capable of reliably generating combinations of chemical and oxygen gradients for cell migration studies, which can be practically utilized in biological labs without sophisticated instrumentation.
08:23
Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry
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Cited by 16 •
2019
Here, we present oxygen gradient ektacytometry, a rapid and reproducible method to measure red blood cell deformability in samples from patients with sickle cell disease under controlled deoxygenation and reoxygenation. This technique provides a way to study red blood cell sickling and to monitor sickle cell disease treatment efficacy.
07:31
Agarose-Based Model Ecosystem for Cultivating Methanotrophs in a Methane-Oxygen Counter Gradient
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2024
A protocol is described for preparing a simple model ecosystem that recreates the methane-oxygen counter gradient found in the natural habitat of aerobic methane-oxidizing bacteria, enabling the study of their physiology in a spatially resolved context. Modifications to common biochemical assays for use with the agarose-based model ecosystem are also described.
08:42
Density Gradient Ultracentrifugation
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2023
Density gradient ultracentrifugation is a common technique used to isolate and purify biomolecules and cell structures. This technique exploits the fact that, in suspension, particles that are more dense than the solvent will sediment, while those that are less dense will float. A high-speed ultracentrifuge is used to accelerate this process in order to separate biomolecules within a density gradient, which can be established by layering liquids of decreasing density in a centrifuge tube. The...
08:58
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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Cited by 28 •
2011
We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.
FAQs
Oxygen profiles in neural tissue emerge from the interaction among delivery, diffusion, and consumption. Blood vessels supply oxygen, the extracellular space provides a route for movement, and neurons and glial cells remove it during mitochondrial respiration. Because these processes vary across tissue, nearby regions can experience different oxygen availability, creating local conditions that shape neural metabolism and responses to stress.
Mitochondrial respiration converts continuous oxygen use into a major driver of local depletion. Neurons and glial cells consume oxygen while sustaining cellular activity, so changes in demand can modify local oxygen profiles even when vascular arrangement remains unchanged. This links cellular energy production to the oxygen environment experienced by surrounding neural tissue and helps explain why metabolic stress can emerge locally.
Vascular structure sets where oxygen enters neural tissue, while diffusion through extracellular space determines how it reaches nearby cells. Their combined effect means oxygen availability is spatially patterned rather than uniform. This matters when interpreting neuronal and glial responses, because a change in vascular organization or tissue-level diffusion can alter the local balance between oxygen delivery and consumption.
Oxygen gradients connect vascular oxygen delivery with the metabolic demands of neural cells, providing a spatial perspective on neurovascular coupling. Examining these profiles helps researchers consider how blood vessels, extracellular diffusion, and neuronal or glial consumption operate together. The resulting information can clarify how changes in neural activity relate to local metabolic conditions within brain tissue.
Disrupted blood flow changes oxygen delivery to neural tissue and can therefore alter local oxygen profiles. Because neurons and glial cells continue consuming oxygen through mitochondrial respiration, an imbalance between supply and demand may increase vulnerability to metabolic stress. This relationship makes oxygen gradients relevant to ischemia, where impaired delivery affects the conditions supporting neural function.
Studying oxygen gradients helps researchers examine how vascular structure, diffusion, and cellular oxygen consumption interact in neural tissue. These measurements or profiles provide context for understanding metabolic stress and the vulnerability of neurons and glial cells. They also support investigation of how altered oxygen conditions may relate to neurodegenerative disorders, alongside broader questions about brain energetics.