Primary Neuron Death
Primary Neuron Death - Related Videos
10:36
Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons
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Cited by 2 •
2017
This protocol describes a simple method for isolating and culturing primary mouse cerebral granule neurons (CGNs) from 6-7 day old pups, efficient transduction of CGNs for loss and gain of function studies, and modelling NMDA-induced neuronal excitotoxicity, low-potassium-induced cell death, DNA-damage, and oxidative stress using the same culture model.
04:25
Neuronal Death Assay to Evaluate Cell Death in Ex Vivo Epilepsy Model
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2025
This video demonstrates the technique of assessing neuronal death in rhinal cortex-hippocampus organotypic slices. Neuronal death occurs via evolving epileptic seizures — resembling in vivo epilepsy — and is detected through dual-staining of the dead cells using propidium iodide and immunofluorescence.
01:59
Modeling Reactive Oxygen Species-Induced Neuronal Death in Mouse Cerebellar Granule Neurons
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2025
This video outlines the generation of a neuronal injury model using reactive oxygen species (ROS) in mouse primary cerebellar granule neurons. It details the process of inducing oxidative stress by adding high concentrations of hydrogen peroxide. ROS exposure causes DNA strand breaks and genomic instability. Additionally, the oxidation of cellular proteins impairs their functions, while lipid peroxidation compromises membrane integrity. This cascade leads to cytochrome c release and caspase...
01:59
Modeling Neuronal Injury in Mouse Primary Cerebellar Granule Neurons
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2025
This video demonstrates the generation of a neuronal injury model in mouse primary cerebellar granule neurons. It outlines the steps involved in inducing neuronal injury using NMDA, an excitatory neurotransmitter receptor agonist, and glycine, a co-agonist. NMDA and glycine bind to specific neuronal receptors, causing their overstimulation and resulting in a massive intracellular calcium influx, which leads to neuronal cell damage and death.
08:43
Primary Neuronal Cultures
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2023
The complexity of the brain often requires neuroscientists to use a simpler system for experimental manipulations and observations. One powerful approach is to generate a primary culture by dissecting nervous system tissue, dissociating it into single cells, and growing those cells in vitro. Primary cultures make neurons and glia easily accessible to the experimental tools required for techniques like genetic manipulation and time-lapse imaging. Furthermore, these cultures represent a highly...
FAQs
Excessive calcium influx can disrupt neuronal homeostasis and contribute to the cascade leading to cell loss. The overview links this influx with energy failure and activation of cellular death pathways, indicating that calcium dysregulation is not an isolated event. Studying this relationship helps researchers connect altered ion handling with downstream neuronal injury in disease and experimental systems.
Programmed cell death and necrosis represent distinct forms of neuronal loss considered in primary neuron death research. Treating them as separate possibilities prevents researchers from interpreting every loss of viability as one uniform mechanism. This distinction supports more focused investigation of how disease, developmental injury, or neurotoxic exposure may produce neuronal damage through different cellular death processes.
Oxidative stress and mitochondrial dysfunction are important because both are identified as contributors to primary neuron death. Mitochondrial dysfunction is closely associated with cellular energy failure, while oxidative stress represents another mechanism that can undermine neuronal viability. Examining these factors helps researchers relate metabolic disruption and cellular damage to the activation of neuronal death pathways.
Researchers measure neuronal death in culture and experimental models to determine whether neurons remain viable and whether survival is preserved under specific conditions. These systems allow investigators to examine cellular mechanisms of loss in a controlled research setting. The resulting observations can connect changes in neuronal viability with disease processes, developmental injury, or neurotoxic exposures.
Studies of primary neuron death provide a framework for investigating neuronal loss in neurodegenerative disease and developmental injury. Researchers can use culture systems and experimental models to examine how cellular death pathways, energy failure, calcium influx, or other listed mechanisms relate to these conditions. This context helps connect cellular observations with broader patterns of nervous-system damage.
Researchers evaluate compounds or other interventions by examining whether they preserve neuronal survival and function in primary neuron death studies. These experiments can also help determine whether an intervention affects mechanisms associated with cellular loss, such as energy failure or activated death pathways. The approach supports testing potential protective strategies in disease, injury, and neurotoxic exposure research.