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Neuroscience

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Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons
 

Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

Article DOI: 10.3791/55871
November 6th, 2017

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Summary November 6th, 2017

This protocol describes a simple method for isolating and culturing primary mouse cerebral granule neurons (CGNs) from 6-7 day old pups, efficient transduction of CGNs for loss and gain of function studies, and modelling NMDA-induced neuronal excitotoxicity, low-potassium-induced cell death, DNA-damage, and oxidative stress using the same culture model.

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