Stereotaxic Infusion
Stereotaxic Infusion - Related Videos
08:16
Stereotaxic Infusion of Oligomeric Amyloid-beta into the Mouse Hippocampus
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Cited by 41 •
2015
Here, we present a protocol for direct stereotaxic brain infusion of amyloid-beta. This methodology provides an alternative in vivo mouse model to address the short-term effects of amyloid-beta on brain neurons.
05:05
Direct Stereotaxic Brain Infusion of Amyloid-Beta Proteins for an Alzheimer's Disease Model
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2025
This video demonstrates the precise stereotaxic infusion of amyloid beta into the dentate gyrus to establish a mouse model of Alzheimer's disease. Accurate coordinate determination and controlled delivery of amyloid beta proteins result in neuronal degradation, disrupting memory processing and contributing to Alzheimer's disease development.
10:19
Neurodegeneration in an Animal Model of Chronic Amyloid-beta Oligomer Infusion Is Counteracted by Antibody Treatment Infused with Osmotic Pumps
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Cited by 11 •
2016
To study the effects of Aβo in vivo, we developed a model based on repeated hippocampal infusions of soluble Aβo coupled with continuous infusion of Aβo antibody (6E10) in the hippocampus using osmotic pumps to counteract the neurotoxic effect of Aβo.
00:08
Survivable Stereotaxic Surgery in Rodents
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Cited by 50 •
2008
The monitoring of extracellular neurotransmitter levels in distinct brain regions of freely moving animals offers insights on the link between neurotransmitter release and behavior. In vivo microdialysis coupled with electrochemical detection provides excellent anatomical and chemical resolution; and information on how basal neurotransmission is altered by pharmacological or physiological manipulations.
08:28
Rodent Stereotaxic Surgery
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2023
Stereotaxic (or stereotactic) surgery is a method used to manipulate the brain of living animals. This technique allows researchers to accurately target deep structures within the brain through the use of a stereotaxic atlas, which provides the 3D coordinates of each area with respect to anatomical landmarks on the skull. After the skull is exposed, anesthetized animals are mounted on a specialized instrument known as a stereotaxic frame, which enables the precise placement of experimental...
FAQs
Researchers identify a target using coordinates referenced to anatomical landmarks in the brain or spinal cord. The stereotaxic apparatus then guides the delivery device according to those coordinates, helping align the infusion with a selected neural region. This spatial reference system is essential when the experiment depends on manipulating a specific circuit rather than neural tissue broadly.
The infused volume and delivery rate influence how far a solution spreads from the intended target. Controlling both variables helps limit exposure of neighboring regions and improves the spatial selectivity of the manipulation. Careful control is therefore important when interpreting behavioral or physiological responses as effects of the selected brain or spinal cord location.
Stereotaxic infusion provides spatially selective delivery to a chosen location, whereas systemic administration does not offer the same direct control over where a solution reaches within the nervous system. This distinction matters when researchers need to associate a drug, tracer, genetic material, or other solution with the function of a particular neural region or circuit.
The technique can deliver drugs, tracers, genetic materials, and other solutions, allowing researchers to pursue different experimental goals. A drug can support localized neural manipulation, a tracer can assist investigations involving neural structures, and genetic material can be used when the study requires targeted delivery of such material. The selected substance determines the type of neural question addressed.
A typical workflow uses a stereotaxic apparatus to identify coordinates relative to anatomical landmarks, guide a needle or cannula through neural tissue, and regulate the volume and rate of delivery. These stages connect anatomical targeting with controlled administration. Together, they help place the solution near the intended brain or spinal cord region while limiting unintended spread.
Researchers choose this approach when an experiment requires localized manipulation that systemic administration cannot provide. It can support studies that compare a targeted neural intervention with resulting behavioral or physiological responses. The method is particularly useful when the research question concerns the contribution of a defined brain or spinal cord location to neural function.
By pairing targeted delivery with measurements of behavior or physiology, researchers can examine how a selected neural location influences function. The approach supports investigations of brain function and disease mechanisms, while also contributing to studies of therapeutic strategies. Its value comes from linking a controlled local intervention with measurable biological or behavioral outcomes.