遗传学，第174期，尿路感染，细菌，杂交基因组组装，纳米孔测序，16S rRNA，下一代测序，移动遗传元件，抗菌素耐药性 Protocols and Video Articles

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Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Authors

Author

Ayelet Di Segni¹, Tzipi Braun¹, Marina BenShoshan¹, Sarit Farage Barhom¹, Efrat Glick Saar¹, Karen Cesarkas¹, James E. Squires², Nathan Keller^1,3, Yael Haberman^1,4

Institution

¹Sheba Medical Center, ²Children's Hospital of Pittsburgh of UPMC, ³Ariel University, ⁴Cincinnati Children's Hospital Medical Center

Journal (Biology)

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

Authors

Author

Lisa Couper¹, Andrea Swei¹

Institution

¹Department of Biology, San Francisco State University

Journal (Biology)

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Authors

Author

Percy Schröttner¹, Florian Gunzer¹, Jana Schüppel², Wolfram W. Rudolph²

Institution

¹Institut für Medizinische Mikrobiologie und Hygiene, Medizinische Fakultät Carl Gustav Carus, TU Dresden, ²Institut für Virologie, Medizinische Fakultät Carl Gustav Carus, TU Dresden

Journal (Immunology and Infection)

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Authors

Author

Shailesh K. Shahi¹, Kasra Zarei², Natalya V. Guseva¹, Ashutosh K. Mangalam^1,2,3,4

Institution

¹Department of Pathology, University of Iowa, ²Medical Scientist Training Program, University of Iowa, ³Graduate Program in Immunology, University of Iowa, ⁴Graduate Program in Molecular Medicine, University of Iowa

Journal (Biology)

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Authors

Author

Melis N. Anahtar¹, Brittany A. Bowman¹, Douglas S. Kwon¹

Institution

¹Ragon Institute of MGH, MIT, and Harvard, Massachusetts General Hospital

Journal (Biology)

Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization

Authors

Author

Michael L. Neugent*¹, Jashkaran Gadhvi*¹, Kelli L. Palmer¹, Philippe E. Zimmern², Nicole J. De Nisco¹

Institution

¹Department of Biological Sciences, University of Texas at Dallas, ²Department of Urology, University of Texas Southwestern Medical Center

Journal (Biology)

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16S rRNA Sequencing: A PCR-based Technique to Identify Bacterial Species

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