Hipsc Cardiac Cells
Hipsc Cardiac Cells - Related Videos
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
06:17
3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells
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Cited by 1 •
2025
A reproducible method is presented to generate 3D myocardial tissues combining melt electrospinning writing (MEW) polycaprolactone (PCL) scaffolds and fibrin hydrogels with hiPSC-derived cardiomyocytes and fibroblasts. This technique offers precise control over scaffold architecture and can be applied in preclinical drug testing and cardiac disease modeling.
11:51
Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
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Cited by 16 •
2016
This manuscript describes the creation of defined engineered cardiac tissues using surface marker expression and cell sorting. The defined tissues can then be used in a multi-tissue bioreactor to investigate mechanisms of cardiac cell therapy in order to provide a functional, yet controlled, model system of the human heart.
08:52
Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection
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Cited by 13 •
2015
This protocol describes how to produce functional sinus nodal tissue from murine pluripotent stem cells (PSC). T-Box3 (TBX3) overexpression plus cardiac Myosin-heavy-chain (Myh6) promoter antibiotic selection leads to highly pure pacemaker cell aggregates. These “Induced-sinoatrial-bodies” (“iSABs”) contain over 80% pacemaker cells, show highly increased beating rates and are able to pace myocardium ex vivo.
07:03
Implantation of hiPSC-derived Cardiac-muscle Patches after Myocardial Injury in a Guinea Pig Model
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Cited by 16 •
2019
Here we present a protocol for the induction of left ventricular cryoinjury followed by the implantation of a cardiac muscle patch, derived from human iPS-cell cardiomyocytes in a guinea pig model.
FAQs
After mature human cells are reprogrammed to pluripotency, researchers expose them to developmental signaling cues that direct cell differentiation toward cardiomyocytes and other cardiac cell types. This staged guidance mirrors key aspects of cardiac development and helps generate biologically relevant populations for studying heart biology, disease mechanisms, and engineered tissue construction.
Defined culture conditions provide a controlled environment during reprogramming and differentiation. By regulating the cellular environment while developmental cues are applied, researchers can guide pluripotent cells toward desired cardiac lineages more deliberately. This control supports reproducible model generation and is important when cells are used for disease studies, drug testing, or tissue engineering.
Cardiomyocytes provide a direct model of heart muscle biology, while additional cardiac cell types can broaden the biological context represented in a model. Generating more than one cardiac lineage allows bioengineers to study cardiac processes in a setting that extends beyond a single cell population, supporting more comprehensive disease modeling and construction of engineered cardiac tissues.
The workflow begins by reprogramming mature human cells so they regain pluripotency. Researchers then apply developmental signaling cues under defined culture conditions to guide differentiation into cardiomyocytes and other cardiac cell types. The resulting cells can be incorporated into laboratory models, three-dimensional systems, or engineered cardiac tissues, depending on the research objective.
These cells are useful when a study requires a renewable human model with a human genetic background. Researchers can apply them to investigate cardiac biology and disease, while also evaluating drug safety and efficacy in laboratory systems. Their compatibility with three-dimensional culture further supports models designed to approximate cardiac behavior more closely.
In bioengineering, hiPSC cardiac cells can be placed within three-dimensional culture systems and used to construct engineered cardiac tissues. Because they retain a human genetic background, they also support patient-relevant research and personalized medicine approaches. These applications connect cell differentiation with regenerative research, while improving the physiological relevance of laboratory cardiac models.