Immune Cell Culture
Immune Cell Culture - Related Videos
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
15:39
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
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Cited by 8 •
2019
Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.
07:36
Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection
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Cited by 1 •
2021
This protocol details an investigation of the early interactions between virally infected nasal epithelial cells and innate cell activation. Individual subsets of immune cells can be distinguished based on their activation in response to viral infections. They can then be further investigated to determine their effects on early antiviral responses.
05:07
Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network
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Cited by 34 •
2016
We have established a technique for the isolation, phenotypic characterization and functional analysis of immune cells from murine gingiva.
07:09
Isolation of Adipose Tissue Immune Cells
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Cited by 74 •
2013
Adipose tissue (AT) is a site of intense immune cell activation and interaction. Almost all cells of the immune system are present in AT and their ratios are altered by obesity. Proper isolation, quantification, and characterization of AT immune cell populations are critical for understanding their role in immunometabolic disease.
FAQs
Cytokines and antigens act as experimental signals that shape what cultured immune cells do. Depending on the study design, they can support survival, trigger activation, promote differentiation, or encourage proliferation. Changing which signal is present, and under what defined conditions, allows investigators to examine how immune cells respond to distinct stimuli rather than treating all cellular behavior as equivalent.
Defined laboratory conditions make it possible to connect a cellular response with selected environmental and signaling variables. Researchers can adjust nutrient-rich media, environmental conditions, cytokines, or antigens and then compare resulting behavior. This control supports reproducible experiments and helps separate responses associated with a particular stimulus from changes caused by less clearly specified culture conditions.
Cells isolated from blood or tissues provide different starting material for investigation, allowing studies to ask whether immune behavior varies with the source of the cells. Once maintained under controlled conditions, researchers can examine survival, activation, differentiation, or proliferation in relation to that starting context. This comparison helps characterize cellular behavior without relying only on observations in the original tissue environment.
An immune cell culture workflow begins with cell isolation from blood or tissue, followed by placement in nutrient-rich culture medium under appropriate environmental conditions. Researchers then introduce selected cytokines or antigens when the experiment requires defined signaling. They assess the resulting cellular behavior to study survival, activation, differentiation, or proliferation while keeping the culture conditions sufficiently controlled for comparison.
By maintaining cells under defined conditions, researchers can examine how selected cytokines or antigens influence cellular behavior and immune responses. The approach provides a controlled setting for connecting an experimental signal with changes such as activation, differentiation, or proliferation. It therefore helps characterize communication processes that may be difficult to interpret when many variables change simultaneously.
These cultures can reveal how immune cells respond to signaling molecules and how cells communicate under defined conditions. Relevant outcomes include changes in survival, activation, differentiation, and proliferation, as well as broader patterns of immune response. Such observations help researchers characterize cellular behavior and connect specific experimental conditions with mechanisms involved in immune biology.
Researchers apply immune cell culture to vaccination, immunotherapy, inflammation, and host-pathogen interaction studies. The same controlled framework can also support investigations of disease mechanisms and evaluations of therapeutic strategies. Its value lies in allowing immune responses and treatment-related effects to be examined in a defined biological system, where experimental conditions can be linked to observed cellular outcomes.