Recombinant Evs
Recombinant Evs - Related Videos
04:56
Determination of the Procoagulant Activity of Extracellular Vesicle (EV) Using EV-Activated Clotting Time (EV-ACT)
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2023
This protocol investigates the use of extracellular vesicle (EV)-rich plasma as an indicator of the coagulative ability of EV. EV-rich plasma is obtained through a process of differential centrifugation and subsequent recalcification.
07:33
Enhancing Chimeric Antigen Receptor-Extracellular Vesicles (CAR-EV) Technology: The Future of Cancer Therapy
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2025
The therapeutic application of extracellular vesicles (EVs) has the potential to revolutionize cancer treatment and drug delivery. Chimeric antigen receptor (CAR) cell-derived EVs (CAR-EVs) isolated using ion-exchange chromatography exhibit increased cargo capacity, significantly enhancing their functional efficacy. This study further characterizes CAR-EVs to elucidate their biological activity and therapeutic potential.
03:22
SDS-PAGE Based Extraction of Extracellular Vesicles Associated Proteins: A Procedure to Extract Proteins from EVs and Prepare Them for In-Gel Digestion
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2023
In this video, we demonstrate the SDS-PAGE in-gel digestion method for protein extraction from extracellular vesicles or EVs. Once isolated, the obtained protein fragments can be used for proteomic analysis.
14:23
Recombineering Homologous Recombination Constructs in Drosophila
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Cited by 9 •
2013
Homologous recombination techniques greatly advance Drosophila genetics by enabling the creation of molecularly precise mutations. The recent adoption of recombineering allows one to manipulate large pieces of DNA and transform them into Drosophila6. The methods presented here combine these techniques to rapidly generate large homologous recombination vectors.
08:56
Recombineering and Gene Targeting
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2023
One of the most widely used tools in modern biology is molecular cloning with restriction enzymes, which create compatible ends between DNA fragments that allow them to be joined together. However, this technique has certain restrictions that limit its applicability for large or complex DNA construct generation. A newer technique that addresses some of these shortcomings is recombineering, which modifies DNA using homologous recombination (HR), the exchange between different DNA molecules based...
FAQs
Cargo location helps determine how a recombinant EV can communicate with recipient cells. Nucleic acids or proteins may be associated with the vesicle lumen, whereas selected proteins or targeting ligands may be presented on the membrane. This distinction allows researchers to compare delivery of internal molecular messages with surface-directed interactions in immune and infection models.
Engineering can occur at two stages: donor cells can be modified before vesicles are produced, or isolated vesicles can be manipulated afterward. Comparing these routes is useful when designing experiments around cargo loading or surface display. The choice also defines whether the main variable is vesicle production by the donor cell or direct vesicle modification.
Recipient-cell internalization is the key downstream event that connects vesicle design to biological effect. Once a cell takes up the vesicle, its associated nucleic acids or proteins can be examined in relation to immune regulation, antigen presentation, or host-pathogen interactions. Uptake studies therefore help link engineered composition with altered cellular communication.
Because researchers can select both the molecular cargo and the surface features, they can build vesicles around a specific immunological question. This tunability supports controlled studies of antigen presentation and immune regulation, while also allowing targeted delivery experiments in which the intended molecular signal or therapeutic molecule is varied.
A basic workflow begins by selecting the intended cargo or surface feature, then choosing whether to modify donor cells or isolated vesicles. The resulting vesicles are examined in a recipient-cell system to determine whether internalization occurs and whether the engineered cargo relates to the response under study. This sequence connects design, delivery, and biological interpretation.
They provide a platform for examining antigen presentation, immune regulation, and host-pathogen interactions without treating vesicle communication as a single fixed process. The same engineering concept also supports vaccine development, diagnostics, and targeted delivery of therapeutic molecules. Their value lies in linking defined vesicle features to research questions across these settings.