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Visualizing the Live Drosophila Glial-neuromuscular Junction with Fluorescent Dyes

Article DOI: 10.3791/1154-v • 10:53 min • May 13th, 2009
May 13th, 2009 •
1, 1, 1
1Department of Zoology, University of British Columbia - UBC

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We described structural features of the Glia-neuromuscular synapses in a novel Inside-out tissue preparation of live fly larvae using fluorescent dyes with confocal microscopy. We labeled live neuron terminals with fluorescent primary antibodies to HRP, and also visualized the perisynaptic space with fluorescent Dextrans.

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Visualizing Live Drosophila Glial-neuromuscular Junction Fluorescent Dyes GFP Labeled Glial Structures Developing Larval Fly Neuromuscular Synapse Live Glial-nerve-muscle Synapses Larval Tissue Preparation Optical Properties Access Of Perfusates Fly Larvae Artificial Hemolymph Rhythmic Body Contractions Dissection Posterior Segments Blunt Insect Pin Everted Larval Body Wall Ultra-fine Dissection Scissors Visceral Side Of Body Wall Muscles Glial Structures At NMJ Membrane Targeted GFP Glial Specific Promoters Post-synaptic Membrane SSR (Subsynaptic Reticula) Muscle Expressed Synaptically Targeted DsRed Acutely Label Motor Neuron Terminals Primary Antibodies To HRP Dye Diffusion Properties Perisynaptic Space
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