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Neuroscience

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JoVE Journal Neuroscience
Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation
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Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation

Article DOI: 10.3791/2821-v • 07:39 min • June 28th, 2011
June 28th, 2011 •
1, 1, 1
1Department of Pathology and Immunology, Washington University School of Medicine

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This protocol describes a simple and inexpensive way to quantify the activity of cis-regulatory elements (i.e., enhancer/promoters) in living mouse retinas via explant electroporation. DNA preparation, retinal dissection, electroporation, retinal explant culture, and post-fixation analysis and quantification are described.

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Cis-regulatory Elements Mouse Retina Explant Electroporation Transcription Factors Gene Networks Enhancers Promoters Transcriptional Activators Transcriptional Repressors Spatiotemporal Pattern Gene Expression Genomic DNA Transgenesis Enhancer Function Quantitative Assays Tissue Culture Systems In Vivo Relevance Electroporation
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