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RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes
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RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes

Article DOI: 10.3791/3479-v • 08:48 min • July 14th, 2012
July 14th, 2012 •
1, 2, 1, 1
1Biology Department, New Mexico State University, 2Molecular Biology Department, New Mexico State University

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In this protocol we combine RNAi-mediated gene silencing with an in vivo diuresis assay to study the effects knockdown of genes of interest has on mosquito fluid excretion.

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RNAi-mediated Gene Knockdown In Vivo Diuresis Assay Aedes Aegypti Mosquitoes Gene Knockdown Technique Bioassay Diuresis In Insects Aquaporin Genes Mosquito Injection Device DsRNA Injection Excretion Rates
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