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Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle
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Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle

Article DOI: 10.3791/4351-v • 07:13 min • January 30th, 2013
January 30th, 2013 •
1, 1
1Max Delbrück Center for Molecular Medicine

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The method presented here comprises the precise injury of live zebrafish embryos with high-energy laser pulses and the subsequent analysis of these injuries and their recovery with time. We also show how genetically labeled single or groups of skeletal muscle cells can be tracked during and after laser light induced damage.

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Zebrafish Embryonic Skeletal Muscle Laser-inflicted Injury Muscle Regeneration Myotoxin Injections Muscle Transplantations Denervation-devascularization Induced Regeneration Intensive Exercise Murine Muscular Dystrophy Models Mdx Mouse Runzel2 Sapje3 Antisense Oligonucleotide Morpholinos Dystrophy-associated Genes Galanthamine Acetylcholinesterase Inhibitor Hypercontraction Laser-mediated Cell Ablation Two-photon Laser
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