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RNAi-mediert Double Gene Knockdown og Gustatory Perception Måling i Honey Bees (
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RNAi-mediert Double Gene Knockdown og Gustatory Perception Måling i Honey Bees (

Article DOI: 10.3791/50446-v • 10:57 min • July 25th, 2013
July 25th, 2013 •
1, 1, 1,2
1School of Life Sciences, Arizona State University, 2Department of Chemistry, Biotechnology, and Food Science, Norwegian University of Life Sciences

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I denne protokollen beskriver vi to strategier som samtidig undertrykker to gener (dobbel genet knockdown) i honningbier. Deretter presenterer vi hvordan du bruker snabel forlengelse respons (PER) analyse for å studere effekten av dobbel genet knockdown på honningbie gustatory oppfatning.

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Nevrovitenskap genetikk Behavior nevrobiologi molekylærbiologi kjemi biokjemi biologi (generelt) genetikk (dyr og planter) dyr biologi RNA-interferens rnai double stranded RNA dsRNA dobbel genet knockdown vitellogenin genet , Ultraspiracle genet, Vitellogenin protein Vg ultraspiracle protein USP grønn fluorescens protein GFP gustatory oppfatning snabel forlengelse respons PER honningbier, Dyremodell assay
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