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A Simplified System for Evaluating Cell Mechanosensing and Durotaxis In Vitro

Article DOI: 10.3791/52949-v • 09:50 min • August 27th, 2015
August 27th, 2015 •
1, 1, 1
1Department of Cell & Developmental Biology, SUNY Upstate Medical University

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Many mammalian cells preferentially migrate towards a more rigid matrix or substrate through durotaxis. The goal of this protocol is to provide a simple in vitro system that can be used to study and manipulate cell durotaxis behaviors by incorporating polydimethylsiloxane (PDMS) substrates of defined rigidity, interfacing with glass coverslips.

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Simplified System Evaluating Cell Mechanosensing Durotaxis In Vitro Extracellular Matrix Tissue Types Connective Tissue Stroma Diversity Cell Behavior Cell Proliferation Differentiation Adhesion Signaling Directional Migration Innate Ability Migration Stiffer Matrix Substrate Compliant Matrix Substrate Embryonic Development Wound Repair Cancer Cell Invasion Assay Polydimethylsiloxane (PDMS) Substrates Rigidity Interface Glass Coverslip Cdc42/Rac1 GTPase Activating Protein (cdGAP) Mechanosensing Regulation
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