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Bovine Mammary Gland Biopsy Techniques

Article DOI: 10.3791/58602-v • 14:54 min • December 23rd, 2018
December 23rd, 2018 •
1,4, 2, 3, 4, 3, 5, 5, 4, 2, 6, 4
1National Animal Nutrition Program, a National Research Support Project (NRSP-9), Department of Animal and Food Sciences, University of Kentucky, 2School of Performing Arts, Virginia Tech, 3Department of Large Animal Clinical Sciences, The Virginia-Maryland College of Veterinary Medicine, Virginia Tech, 4Department of Dairy Science, Virginia Tech, 5Department of Animal Biosciences, University of Guelph, 6School of Visual Arts, Virginia Tech

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This article presents a bovine mammary gland biopsy using core and needle biopsy tools. Harvested tissue can be used for cell culture or to assess mammary physiology and metabolism including gene expression, protein expression, protein modifications, immunohistochemistry, and metabolite concentrations.

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Biopsy Mammary Gland Tissue Sampling Gene Expression Analysis Histological Analysis Microscopic Level Signaling Pathways Hormone Action Representative Sample Lobule Alveoli Parenchymal Tissue Milk Production Sensory Nerve Endings Skin Preparation Incision Anesthesia
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