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Imunologia e Infecção
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Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients
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Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients

DOI:
10.3791/61631-v

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07:25 min

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July 16, 2020

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, , , , ,
1Translational Medicine,Hospital for Sick Children, 2Center for Microbial Pathogenesis, Department of Pediatrics,University of Pittsburgh, 3Microbiology, Department of Pediatric Laboratory Medicine,Hospital for Sick Children, 4Infectious Diseases, Department of Pediatrics,Hospital for Sick Children

Summary

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Tadução automática

This protocol provides methods for visualization of bacterial cells and polysaccharide synthesis locus (Psl) polysaccharide within the sputum of cystic fibrosis patients.

Tags

Pseudomonas AeruginosaCystic FibrosisSputumVisualizationHydrogelExopolysaccharidePslBiofilmAntimicrobial SusceptibilityFixationAcrylamideAnaerobicSDSClearing

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