Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / Subculture and Cryopreservation of Esophageal…
JoVE Journal
Cancer Research

É necessário ter uma assinatura JoVE para assistir este

JoVE Journal Cancer Research
Subculture and Cryopreservation of Esophageal Adenocarcinoma Organoids
Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts Isolated from 3D Multicellular Lung Tumor Spheroids
Journal (Cancer Research)
Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts Isolated from 3D Multicellular Lung Tumor Spheroids
Southern Blotting, Hybridization, and Chemiluminescence Detection of Genomic DNA Isolated from A2780 Cells (Video) | JoVE
Journal (Cancer Research)
Southern Blotting, Hybridization, and Chemiluminescence Detection of Genomic DNA Isolated from A2780 Cells (Video) | JoVE
 

Subculture and Cryopreservation of Esophageal Adenocarcinoma Organoids: Pros and Cons for Single Cell Digestion

Article DOI: 10.3791/63281-v • 10:42 min • July 6th, 2022
July 6th, 2022 •
*1, *1, 1, 2, 1, 1
1Department of General, Visceral, Cancer and Transplantation Surgery, University Hospital Cologne, 2Institute of Pathology, University Hospital Cologne
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

This protocol describes the methods of subculture and cryopreservation of esophageal adenocarcinoma organoids with and without single cell digestion to enable researchers to choose appropriate strategies based on their experimental design.

Tags

Subculture Cryopreservation Esophageal Adenocarcinoma Organoids Single Cell Digestion Protocol Handling Subculture Process Downstream Applications PDO-based Experiments Details Quality Control Team-based SOP PhD Student Laboratory Technique Assistant Incubation ECM Gel Cell Recovery Solution
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter