In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

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JoVE Journal
Medicine

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JoVE Journal Medicine

In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

Article DOI: 10.3791/64227-v • 09:17 min • September 7th, 2022

September 7th, 2022 •

This protocol describes the procedure for measuring ATP concentrations in the lumen of the bladder in an anesthetized rodent.

In Vivo Luminal Measurement Distension-Evoked Urothelial ATP Release Rodents ATP Transmitter Urinary Bladder Mechanisms Controlling Release Physiological Relevance Cultured Cells Bladder Tissue Keara Healy Stephanie Daugherty Research Technicians Anesthesia Closed Box Isoflurane Oxygen Urethane Administration Proper Plane Of Anesthesia Forceps Bladder Contraction Prevention Ganglionic Blocking Agent Hexamethonium Ophthalmic Ointment Shave Abdomen Midline Laparotomy Urinary Bladder Exposure PE50 Intramedic Tubing Flame Sterilization Krebs Solution Silk Suture