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Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry
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Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Article DOI: 10.3791/64572-v • 09:16 min • February 3rd, 2023
February 3rd, 2023 •
*1,2, *1, 1, 1,2, 1,2, 1,2
1Schepens Eye Research Institute of Mass. Eye and Ear, 2Department of Ophthalmology, Harvard Medical School
* These authors contributed equally

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The metabolic status of human retinal pigment epithelial cells (H-RPE) reflects their health and function. Presented here is an optimized protocol for examining the real-time metabolic flux of H-RPE using high-resolution respirometry.

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Real-time Analysis Bioenergetics Primary Human Retinal Pigment Epithelial Cells High-resolution Respirometry Metabolic Status Oxphos Glycolysis Oxygen Consumption Rate OCR Extracellular Acidification Rate ECAR AMD Mitochondrial Function Cell Suspension Human RPE Medium Background Correction Cell Culture Plate Morphology Pigment
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