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Visualization of Organelles In Situ by Cryo-STEM Tomography
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Visualization of Organelles In Situ by Cryo-STEM Tomography

Article DOI: 10.3791/65052-v • 08:37 min • June 23rd, 2023
June 23rd, 2023 •
1, 1, 2, 1
1Department of Chemical and Biological Physics, Weizmann Institute of Sciences, 2Department of Chemical Research Support, Weizmann Institute of Sciences

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Cryo-STEM tomography provides a means to visualize organelles of intact cells without embedding, sectioning, or other invasive preparations. The 3D resolution obtained is currently in the range of a few nanometers, with a field of view of several micrometers and an accessible thickness in the order of 1 µm.

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STEM Tomography Cryo-STEM Tomography TEM Tomography FIB SEM Soft X-ray Tomography Specimen Thickness Resolution Cryogenic Preparation Biological Samples 3D Sections STEM Optics Image Formation Service Engineer Alignment Lomax Stem Modes Calibration Steps Side-entry Cryo Holder TEM Mode Eucentric Focus Spot Size Fluorescent Screen Visualization Microscope Settings
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