Rodent Kidney Scaffold
Rodent Kidney Scaffold - Related Videos
03:30
Preparation of Decellularized Rat Kidney: A Procedure to Generate Acellular Vascularized Whole-organ Scaffold from a Harvested Rat Kidney
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2023
This video describes the preparation of a decellularized rat kidney. The obtained acellular vascular scaffold can serve as a promising platform for tissue engineering of organ grafts.
09:43
Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
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Cited by 17 •
2015
This protocol describes decellularization of Sprague Dawley rat kidneys by antegrade perfusion of detergents through the vasculature, producing acellular renal extracellular matrices that serve as templates for repopulation with human renal epithelial cells. Recellularization and use of the resazurin perfusion assay to monitor growth is performed within specially-designed perfusion bioreactors.
06:09
Preparation of Decellularized Kidney Scaffolds in Rats
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Cited by 1 •
2021
This protocol introduces a method to develop a scaffold using decellularized rat kidneys. The protocol includes decellularization and recellularization processes to confirm bioavailability. Decellularization is performed using Triton X-100 and sodium dodecyl sulfate.
07:35
Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring
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Cited by 14 •
2015
The use of ultra-high field MRI as a non-invasive way to obtain phenotypic information of rodent models for polycystic kidney disease and to monitor interventions is described. Compared with the traditional histological approach, MRI images can be acquired in vivo, allowing for longitudinal follow-up.
08:19
The Mouse Isolated Perfused Kidney Technique
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Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
FAQs
Decellularization must remove cellular material without destroying the kidney’s structural framework. Detergents and perfusion-based delivery support this balance by exposing the organ throughout its vascular pathways. Preserving matrix proteins and spatial organization gives later experiments a reproducible three-dimensional setting in which seeded cells can interact with renal architecture rather than with an unstructured material.
The preserved vascular network and tubular architecture retain spatial relationships that are difficult to reproduce in simpler culture systems. These features allow researchers to examine how renal or vascular cells attach within organized compartments and to study perfusion through an anatomically arranged framework. The scaffold therefore provides structural context for evaluating tissue remodeling and engineered kidney development.
Cell attachment, differentiation, and tissue remodeling provide distinct readouts of scaffold performance. Attachment indicates whether cells can associate with the preserved matrix, while differentiation shows whether the environment supports more specialized cell behavior. Remodeling reveals how cells alter or reorganize the framework over time. Together, these outcomes help assess the scaffold’s regenerative potential under controlled experimental conditions.
A typical workflow begins with perfusion-based decellularization and detergent treatment to remove cellular material while retaining matrix structure, vascular organization, and tubules. Researchers then introduce renal or vascular cells onto the prepared framework. Subsequent experiments examine cell attachment, differentiation, perfusion, and remodeling under controlled conditions, allowing the scaffold and its seeded cells to be evaluated together.
This platform is useful when investigators need an organized kidney-derived framework for testing regeneration or organ-building strategies. It can support studies of engineered kidney tissue, disease mechanisms, and drug responses. Because researchers can seed defined renal or vascular cells and monitor their behavior, the model also helps investigate why rebuilding a functional organ remains difficult.
A rodent kidney scaffold can show how cells behave within preserved renal architecture rather than in a simplified environment. Measurements of attachment, differentiation, perfusion, and tissue remodeling provide evidence about how closely engineered tissue responds to an organ-like framework. In bioengineering, these observations help identify challenges in restoring coordinated kidney structure and function.