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Preparation of Decellularized Kidney Scaffolds in Rats
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Preparation of Decellularized Kidney Scaffolds in Rats

Preparation of Decellularized Kidney Scaffolds in Rats

DOI:
10.3791/61856-v

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06:09 min

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March 18, 2021

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, , , , , , ,
1Department of Internal Medicine and Biomedical Research Institute,Pusan National University Hospital, 2Biomedical Research Institute,Pusan National University Hospital, 3Department of Plastic Surgery,Pusan National University Hospital, 4Department of Otorhinolaryngology – Head and Neck Surgery,Yangsan Pusan National University Hospital, 5Department of Otorhinolaryngology – Head and Neck Surgery,Pusan National University Hospital, 6Department of Pathology,Pusan National University Hospital

Chapters

  • 00:04Introduction
  • 01:00Preparation for Harvesting Rat Kidneys
  • 01:55Transcardial Perfusion
  • 03:22Kidney Harvesting and Decellularization
  • 04:37Results: Rat Kidneys Subjected to Renal Arterial Perfusion Decellularization
  • 05:35Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol introduces a method to develop a scaffold using decellularized rat kidneys. The protocol includes decellularization and recellularization processes to confirm bioavailability. Decellularization is performed using Triton X-100 and sodium dodecyl sulfate.

Tags

Keywords: DecellularizationKidney ScaffoldVascularized ScaffoldStem Cell DifferentiationTransplantation MedicineSprague Dawley RatPerfusionPBSHeparinPFAAbdominal AortaInferior Vena CavaKidney Harvest

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