Human Dental Epithelial Cells
Human Dental Epithelial Cells - Related Videos
04:07
Isolation of Epithelial Cells from Human Dental Follicle
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Cited by 1 •
2021
The dental follicle contains an epithelial population and mesenchymal cells. The epithelial population was selected from the heterogeneous dental follicle cell population by providing a distinct culture medium. Epithelial cells survived and formed colonies in a serum-free medium.
08:14
Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
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Cited by 24 •
2014
The continuously growing mouse incisor provides a model for studying renewal of dental tissues from dental epithelial stem cells (DESCs). A robust system for consistently and reliably obtaining these cells from the incisor and expanding them in vitro is reported here.
14:52
Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
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Cited by 100 •
2012
The method described isolation and characterization of human Dental Pulp Stem Cells (hDPSCs) by using either enzymatic dissociation of pulp (DPSC-ED) or direct outgrowth of stem cells from pulp tissue explants (DPSC-OG). Then followed by in vitro comparative differentiation of both types of hDPSCs into odontoblasts.
14:32
Primary Human Bronchial Epithelial Cells Grown from Explants
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Cited by 19 •
2010
Here we describe a detailed method for growing primary human bronchial epithelial cells from explants of human bronchial airway tissue including differentiated growth on an air-liquid interface. This method provides an abundant source of primary cells for investigating the role of the airway epithelium in human lung health and disease.
09:07
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
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Cited by 27 •
2013
This protocol details a method to isolate antigen presenting cells from human thymus via different steps of enzymatic digestion of the tissue followed by density centrifugation of the single cell suspension and finally magnetic and/or FACS sorting of the cell populations of interest.
FAQs
Cell polarity organizes the direction in which human dental epithelial cells receive signals, maintain contacts, and interact with neighboring tissues. This organization supports coordinated proliferation and differentiation rather than random cell behavior. In tooth-related models, examining polarity helps researchers connect epithelial organization with tissue architecture and with the development of specialized enamel-forming cell types.
Cell-cell adhesion keeps epithelial cells organized into connected tissue structures, while basement-membrane interactions anchor them and provide environmental cues. Together, these relationships influence polarity, proliferation, and differentiation. Altering either interaction can therefore change how cells organize in culture, making these features useful for studying tissue formation and epithelial responses to injury or disease.
Signaling pathways regulate whether human dental epithelial cells continue proliferating, begin differentiating, or maintain an organized epithelial state. Their effects are interpreted alongside cell polarity, adhesion, and basement-membrane contact because these features shape how signals are received and transmitted. Studying these combined influences helps explain enamel-forming cell development and tissue organization during odontogenesis.
Epithelial-mesenchymal interactions provide coordinated signals between dental epithelial cells and surrounding mesenchymal tissues. These interactions can guide epithelial organization and differentiation, making them central to models of odontogenesis and tooth-like structure formation. In regenerative biology, controlling this communication helps researchers investigate how tissue components cooperate instead of evaluating epithelial cells in isolation.
Laboratory culture provides a controlled model for examining dental epithelial behavior, including organization, proliferation, differentiation, and responses to injury or disease. Researchers can focus on how polarity, cell-cell adhesion, basement-membrane interactions, and signaling affect cellular outcomes. These observations help connect cell-level behavior with broader questions about tooth development and oral tissue biology.
They are useful when researchers need to examine how epithelial and mesenchymal components can be coordinated during regenerative tissue formation. Human dental epithelial cells support investigations into controlled epithelial-mesenchymal interactions and the formation of tooth-like structures. This work may improve understanding of the biological requirements for organized dental tissue development and regeneration.
These cells support studies of odontogenesis, enamel-forming cell development, epithelial barrier function, and tissue responses to injury or disease. Their behavior also provides a way to examine how organization and signaling contribute to oral tissue health. As a result, culture models can link fundamental biology with dental regenerative research and investigations of oral disease processes.