Odonata Gene Suppression

Odonata gene suppression is the experimental reduction of a selected gene’s activity in dragonflies and damselflies, enabling researchers to test how genes control development. The approach interferes with gene expression or the production of its functional product, then compares altered individuals with untreated controls to identify resulting developmental changes. In developmental biology, these experiments help link specific genes to embryonic patterning, growth, metamorphosis, and the formation of specialized structures. Applying gene suppression to Odonata also provides insight into how developmental programs evolve across insects and supports comparative studies of traits that distinguish aquatic juvenile stages from winged adults.

Odonata Gene Suppression - Related Videos

Research

JoVE Journal - Medicine
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The Measurement and Treatment of Suppression in Amblyopia

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Cited by 54 •

2012

Amblyopia is a developmental disorder of the visual cortex that is often accompanied by strong suppression of one eye. We present a new technique for measuring and treating interocular suppression in patients with amblyopia that can be deployed using virtual reality goggles or a portable iPod Touch device.

Electroporation-mediated RNA Interference Method in Odonata

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Cited by 8 •

2021

We provide a detailed protocol for electroporation-mediated RNA interference in insects of the order Odonata (dragonflies and damselflies) using the blue-tailed damselfly (Ischnura senegalensis: Coenagironidae: Zygoptera) and the pied skimmer dragonfly (Pseudothemis zonata: Libellulidae: Anisoptera).

Research

JoVE EoE - Viral Growth and Techniques

Visualizing Transcriptional Suppression in Virus-Infected Cells

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2026

Source: Kalveram, B. et al. Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis. J. Vis. Exp. (2013)This video demonstrates the use of click chemistry and fluorescence microscopy to visualize nascent RNA transcription in mammalian cells. It highlights how viral infection suppresses host transcription by comparing fluorescent signals between infected and control cells.

Co-Infiltration Assay for Screening Pathogen Effectors that Suppress Plant RNA Silencing

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2026

Source: Shi, J., et al. Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens. J. Vis. Exp. (2020)This video demonstrates a co-infiltration assay for identifying pathogen effector proteins that suppress RNA silencing in Nicotiana benthamiana 16c. A fluorescent reporter and a candidate effector gene are delivered into plant leaves via Agrobacterium. Normally, RNA silencing degrades reporter transcripts, reducing fluorescence. Suppressor effectors...

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance

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2011

Tregs are potent suppressors of the immune system. There is a lack of unique surface markers to define them, hence, definitions of Tregs are primarily functional. Here we describe an optimized in vitro assay capable of identifying immune imbalance in subjects at risk to develop T1D.

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