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Electroporation-mediated RNA Interference Method in Odonata
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Developmental Biology
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JoVE Journal Developmental Biology
Electroporation-mediated RNA Interference Method in Odonata

Electroporation-mediated RNA Interference Method in Odonata

DOI:
10.3791/61952-v

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13:28 min

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February 06, 2021

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, ,
1Department of Biological Sciences, Graduate School of Science,The University of Tokyo, 2Bioproduction Research Institute,National Institute of Advanced Industrial Science and Technology (AIST), 3Graduate School of Life and Environmental Sciences,University of Tsukuba

Chapters

  • 00:00Introduction
  • 01:01Protocol: Electroporation-mediated RNAi- In the Abdomen of Damselfly (Ischnura senegalensis)
  • 04:21Protocol: Electroporation-mediated RNAi- In the Thorax of Damselfly (Ischnura senegalensis)
  • 06:54Protocol: Electroporation-mediated RNAi- In the Abdomen of Dragonfly (Pseudothemis zonata)
  • 10:16Representative Results
  • 11:51Discussion

Summary

Automatic Translation

Automatic Translation

We provide a detailed protocol for electroporation-mediated RNA interference in insects of the order Odonata (dragonflies and damselflies) using the blue-tailed damselfly (Ischnura senegalensis: Coenagironidae: Zygoptera) and the pied skimmer dragonfly (Pseudothemis zonata: Libellulidae: Anisoptera).

Tags

Keywords: ElectroporationRNA InterferenceRNAiOdonataDragonflyDamselflyIschnura SenegalensisGene Function AnalysisIn VivoProthoraxAbdomenSiRNADsRNAElectroporator

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