Subcellular Structure Dynamics
Subcellular Structure Dynamics - Related Videos
11:19
Imaging Subcellular Structures in the Living Zebrafish Embryo
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Cited by 8 •
2016
Imaging the dynamic behavior of organelles and other subcellular structures in vivo can shed light on their function in physiological and disease conditions. Here, we present methods for genetically tagging two organelles, centrosomes and mitochondria, and imaging their dynamics in living zebrafish embryos using wide-field and confocal microscopy.
12:03
Dynamics of Structures
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2023
Source: Roberto Leon, Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA It is rare nowadays that a whole year goes by without a major earthquake event wreaking havoc somewhere around the world. In some cases, like the 2005 Banda Ache earthquake in Indonesia, the damage involved large geographic areas and casualties in the six figures. In general, the number and intensity of earthquakes is not increasing, however, the vulnerability of the built environment is...
03:17
Imaging Subcellular Calcium Dynamics in Neurons of Caenorhabditis elegans
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2025
Source: Doser, R., et al. Subcellular Imaging of Neuronal Calcium Handling In Vivo. J. Vis. Exp. (2023)This video demonstrates in vivo imaging of subcellular calcium flux in the ventral nerve cord neurons of transgenic Caenorhabditis elegans strains. Two distinct calcium indicators are employed: a cytoplasmic indicator in one strain and a mitochondrial indicator in another. Neurons are observed under a fluorescence microscope to monitor real-time changes in fluorescence intensity within the...
08:23
Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
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Cited by 18 •
2013
Intravital microscopy is a powerful tool that enables imaging various biological processes in live animals. In this article, we present a detailed method for imaging the dynamics of subcellular structures, such as the secretory granules, in the salivary glands of live mice.
08:17
Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
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Cited by 1 •
2021
Imaging-derived mean square displacement (iMSD) analysis is applied to macropinosomes to highlight their intrinsic time-evolving nature in terms of structural and dynamic properties. Macropinosomes are then compared to insulin secretory granules (ISGs) as a reference for subcellular structures with time-invariant average structural/dynamic properties.
FAQs
Protein assembly and disassembly allow molecular structures to form, reorganize, and separate at appropriate times. This turnover can change the architecture of organelles, cytoskeletal elements, or other molecular assemblies as developmental cues alter cellular requirements. By controlling when structures persist or are dismantled, cells can adjust their internal organization during processes such as division, migration, and differentiation.
Membrane remodeling changes the shape and connectivity of cellular membranes, while motor-protein transport changes the position of selected materials within the cell. Together, these processes redistribute internal components and alter their interactions. Their coordinated activity helps establish spatial organization needed for developmental behaviors, including polarity, cell movement, and the orderly formation of tissues.
Regulated signaling connects developmental cues to changes in cellular architecture. Signals can influence protein assembly, membrane remodeling, transport, or the interactions among molecular assemblies, allowing internal structures to respond rather than remain fixed. This coordination is important because the same cell may need different spatial arrangements as it divides, migrates, or adopts a differentiated state.
Live-cell imaging follows changes in subcellular structures over time instead of capturing only a fixed state. It can show when organelles, cytoskeletal elements, membranes, or molecular assemblies change position, organization, or interactions. In developmental studies, these observations help relate structural changes to cell polarity, division, migration, and differentiation as they occur.
Molecular perturbation changes selected molecular components or regulatory processes so researchers can examine how internal organization responds. Comparing altered and normal cells can identify effects on structure position, interactions, or timing. This approach is especially useful for investigating how disrupted dynamics contribute to abnormal development and disease, complementing observations from imaging and live-cell analysis.
During tissue formation, cells must coordinate internal architecture with changing developmental instructions. Dynamic control supports polarity, helps organize division, guides migration, and enables differentiation, linking subcellular behavior to larger-scale tissue development. Studying these relationships can clarify how normal tissues arise and how failures in organelle, membrane, cytoskeletal, or molecular-assembly dynamics may produce developmental abnormalities.