Stark Effect

The Stark effect is the shifting or splitting of atomic and molecular spectral lines when an external electric field perturbs energy levels, providing a way to probe matter and electromagnetic environments. The field interacts with permanent or induced electric dipole moments, changing the energies of quantum states and producing linear or quadratic changes in transition frequencies depending on the system and field strength. In engineering, Stark-effect measurements support precision spectroscopy, electric-field sensing, plasma diagnostics, and the design of quantum and optoelectronic devices. The effect also helps researchers characterize materials, control light-matter interactions, and develop tunable components for communications and photonics.

Stark Effect - Related Videos

Education

JoVE Science Education - Chemistry

Dean-Stark Trap

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2023

Source: Vy M. Dong and Jan Riedel, Department of Chemistry, University of California, Irvine, CA A Dean-Stark trap is a special piece of glassware, which allows the collection of water during a reaction through an azeotropic distillation. The desire to collect water from a reaction can have various reasons. It can drive the equilibria in reactions, where water is formed as a byproduct. According to Le Chatelier's principle, a change in temperature, pressure, concentration, or volume will cause...

Research

JoVE Journal - Biology
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Isolation and Biophysical Study of Fruit Cuticles

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Cited by 16 •

2012

Aerial plant organs are protected by the cuticle, a supramolecular biopolyester-wax assembly. We present protocols to monitor selective removal of epi- and intracuticular waxes from tomato fruit cuticles on molecular and micro scales by solid-state NMR and atomic force microscopy, respectively, and to assess the cross-linking capacity of engineered cuticular biopolyesters.

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

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Cited by 8 •

2018

A protocol for the isolation of primary microglia from murine brains is presented. This technique aids in furthering the current understanding of neurological conditions. Density gradient centrifugation and magnetic separation are combined to produce sufficient yield of a highly pure sample. Furthermore, we outline the steps for characterization of microglia.

Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods

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Cited by 15 •

2018

We have engineered the capsid protein of hepatitis E virus as a theranostic nanoparticle (HEVNP). HEVNP self-assembles into a stable icosahedral cage in mucosal delivery. Here, we describe the modification of HEVNPs for tumor targeting by mutating surface-exposed residues to cysteines, which conjugate synthetic ligands that specifically bind tumor cells.

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

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Cited by 15 •

2015

Studying medullary thymic epithelial cells in vitro has been largely unsuccessful, as current 2D culture systems do not mimic the in vivo scenario. The 3D culture system described herein - a modified skin organotypic culture model - has proven superior in recapitulating mTEC proliferation, differentiation and maintenance of promiscuous gene expression.

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