Marine Lipid Analysis

Marine lipid analysis is the study of fats and other lipid molecules in seawater, sediments, and marine organisms to characterize ecosystem composition and change. It typically involves extracting lipids from environmental samples, separating them by chemical properties, and identifying or quantifying compounds such as fatty acids, sterols, and phospholipids using chromatographic and spectrometric methods. These molecular profiles can reveal dietary relationships, trophic transfer, organismal condition, and shifts caused by pollution, climate change, or other environmental pressures. By linking lipid biomarkers with biological and geochemical processes, marine lipid analysis supports ecosystem monitoring, food-web research, biogeochemical studies, and assessment of ocean health.

Marine Lipid Analysis - Related Videos

Research

JoVE Journal - Environment

Determination of Total Lipid and Lipid Classes in Marine Samples

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Cited by 3 •

2021

This protocol is for the determination of lipids in seawater and biological specimens. Lipids in filtrates are extracted with chloroform or mixtures of chloroform and methanol in the case of solids. Lipid classes are measured by rod thin-layer chromatography with flame ionization detection and their sum gives the total lipid content.

Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

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Cited by 60 •

2009

We present a method for generating cDNA from environmental mRNA. In general, total RNA is first collected from the environment, rRNA is selectively removed, mRNA is selectively amplified, and cDNA synthesized from the enriched mRNA pool is sequenced. Recovered sequences can be annotated using standard bioinformatics techniques to identify the expressed genes.

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

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Cited by 7 •

2017

Lipid droplets are important organelles for the replication of several pathogens, including the Hepatitis C Virus (HCV). We describe a method to isolate lipid droplets for quantitative mass spectrometry of associated proteins; it can be used under a variety of conditions, such as virus infection, environmental stress, or drug treatment.

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm

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Cited by 2 •

2017

We demonstrate the use of various microscopy methods that are useful in observing the calcification of a tubeworm, Hydroides elegans, as well as locating and characterizing the first calcified material. Live microscopy and electron microscopy are used together to provide functional and material information that are important in studying biomineralization.

Education

JoVE Science Education - Environmental Sciences

Sonication Extraction of Lipid Biomarkers from Sediment

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2023

Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst The material comprising the living "organic" share of any ecosystem (leaves, fungi, bark, tissue; Figure 1) differs fundamentally from the material of the non-living "inorganic" share (rocks and their constituent minerals, oxygen, water, metals). Organic material contains carbon linked to a series of other carbon and hydrogen molecules (Figure 2), which distinguishes it from inorganic material. Carbon's wide valency range...

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