Everted Gut Sac
Everted Gut Sac - Related Videos
10:38
Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM
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Cited by 19 •
2008
This video shows how to micro-dissect the yolk sac and aorta-gonad-mesonephros region from embryos and use flow cytometry to sort hematopoietic stem cells.
15:27
Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes
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Cited by 18 •
2007
This video illustrates the technique for extracting DNA from the species of microbes resident in the termite hindgut. The preparation of a wet mount slide, which is useful for visualizing the gut microbial community is also illustrated, and a tour through the species-rich gut environment is given.
04:34
Surgical Treatment of an Endolymphatic Sac Tumor
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Cited by 1 •
2023
Here, we discuss clinical and radiological features of endolymphatic sac tumors and report the methodology and results of a surgical removal in a case in care at our otologic referral center.
11:53
Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
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Cited by 6 •
2016
To study the mechanism of lipid utilization in yolk sac membranes during the late stages of avian embryonic development, we established a primary Japanese quail embryonic endodermal epithelial cell culture system.
04:34
Dissection of the Endolymphatic Sac from Mice
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Cited by 5 •
2021
This study describes how to dissect the endolymphatic sac from the inner ear of the mouse at different ages. The result of a similar dissection is shown in an Slc26a4-null mouse model of enlargement of the endolymphatic sac. A transgenic mouse with a fluorescent reporter expressed in the endolymphatic sac is presented as a model to readily visualize the endolymphatic sac, and educational tool.
FAQs
Turning the intestinal segment inside out creates a defined experimental path between the mucosal and serosal sides. The test compound is placed outside the sac, while the internal oxygenated buffer represents the receiving compartment. Any change detected inside therefore provides an indicator of movement across the gut wall rather than simple exposure of the tissue to the compound.
Transporter activity can influence how efficiently a nutrient, drug, or ion crosses the intestinal tissue, whereas epithelial integrity affects whether the barrier remains functionally selective. Measuring absorption while considering both factors helps separate regulated transport from changes associated with altered tissue condition, making the model useful for linking intestinal function to underlying biological mechanisms.
Genetic variation can alter molecular features that influence intestinal transport or epithelial behavior. The Everted Gut Sac connects those gene-dependent differences with a measurable change in material appearing inside the sac. This relationship gives genetics researchers a way to examine how molecular variation may produce a physiological difference in intestinal absorption under controlled conditions.
A typical setup uses an intestinal segment that is carefully turned inside out, filled with oxygenated buffer, and placed in a solution containing the substance under investigation. Researchers then monitor changes in the internal compartment. Keeping the orientation, internal medium, external solution, and observation period consistent is important for comparing transport outcomes across experimental conditions.
Changes in the material collected or detected inside the sac indicate transfer from the mucosal side toward the serosal side. Depending on the experimental substance, the resulting measurement can represent absorption of a nutrient, drug, or ion. These outcomes allow investigators to compare transport behavior while examining influences such as epithelial integrity, transporter function, or genetic variation.
In genetics, the model is used to compare intestinal absorption when genetic differences are expected to affect transport-related processes or epithelial function. Researchers can relate a molecular change or gene-dependent phenotype to a measurable transport outcome. This provides subject-specific physiological context, helping connect genetic information with experimentally observed differences in gut function.