Antibody Amplification System
Antibody Amplification System - Related Videos
09:44
Rapid Amplification of cDNA Ends
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2023
Source: Pablo Sanchez Bosch2, Sean Corcoran2 and Katja Brückner1,2,3 1Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research 2Department of Cell and Tissue Biology, 3Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA Rapid Amplification of cDNA Ends (RACE) is a technique that allows amplification of full-length cDNA from mRNA by extending to the 3’ or 5’ end, even without prior knowledge of the sequence (Frohman et al.,...
10:12
Protein Misfolding Cyclic Amplification of Prions
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Cited by 7 •
2012
Protein misfolding cyclic amplification (PMCA) is an in vitro assay for the study of prion conversion and strain and species barriers. It can also be used as a prion detection assay.
12:55
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
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Cited by 33 •
2015
A method is described with visual accompaniment for conducting scalable, high throughput selections from phage-displayed combinatorial synthetic antibody libraries against hundreds of antigens simultaneously. Using this parallel approach, we have isolated antibody fragments that exhibit high affinity and specificity for diverse antigens that are functional in standard immunoassays.
02:41
Amplification of a Suicide Plasmid in a Bacterial Host
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2025
Source: Tomás, J. M., et al. Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility. J. Vis. Exp. (2022).This video demonstrates the initiator-dependent amplification of a suicide plasmid in E. coli. The plasmid, which carries an antibiotic resistance gene, replicates only in the presence of an initiator protein encoded by the host. After electroporation and incubation in recovery medium, the mixture is plated on antibiotic-containing agar.
09:36
Visualization of Mitochondrial DNA Replication in Individual Cells by EdU Signal Amplification
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Cited by 11 •
2010
We developed a sensitive technique to label newly synthesized mitochondrial DNA (mtDNA) in individual cells in order to study mtDNA biogenesis. The technique combines the incorporation of EdU together with a tyramide signal amplification (TSA) protocol to visualize mtDNA replication within subcellular compartments of neurons.
FAQs
Signal layering increases detectability by adding labeled secondary antibodies or other affinity-based reagents after the primary antibody has bound the target. These added components carry enzymes or fluorophores that generate the measurable output. Consequently, a target-bound primary antibody can produce a stronger visual or detectable signal than direct labeling alone, which is especially useful for low-abundance targets.
Secondary antibodies and affinity-based reagents connect the target-bound primary antibody to the signal-generating component. Their binding provides an additional layer between antigen recognition and detection, allowing enzymes or fluorophores to contribute to the readout. This arrangement is central to amplification because the system does not rely solely on a label attached directly to the primary antibody.
Direct labeling may produce a weak readout when the target is present at low abundance. An amplification system addresses this limitation by layering labeled reagents onto the primary antibody, increasing the measurable signal associated with the binding event. The resulting improvement in visualization can help researchers identify targets that might be difficult to observe with direct labeling.
The detection sequence begins when a primary antibody binds the antigen in the sample. A labeled secondary antibody or affinity-based reagent then binds the primary antibody. Finally, an attached enzyme or fluorophore generates the measurable signal. This sequence can be applied to immunological or infectious-disease samples when the target requires enhanced visualization.
The approach supports antigen detection in cells, tissues, and diagnostic specimens. In each setting, the layered reagents improve visualization of the antigen-antibody interaction when direct labeling produces insufficient signal. This makes the method relevant to both laboratory examination of biological material and analysis of specimens used in immunological or infectious-disease research.
In immunology and infection research, stronger detection can help reveal antigens in biological samples where target abundance is limited. Researchers can use the amplified readout to visualize targets in cells or tissues and to examine diagnostic specimens. Its main contribution is improved observability of antigen-antibody binding, supporting studies that depend on clear target detection.