Cd11b Cells
Cd11b Cells - Related Videos
07:54
Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility
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Cited by 22 •
2017
Here, we present a protocol to isolate microglia from postnatal mouse pups (day 1) for in vitro experimentation. This improvised method of isolation generates both high yield and purity, a significant advantage over alternate methods that allows broad range experimentation for the purposes of elucidating microglial biology.
12:14
Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
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Cited by 80 •
2016
Inflammation plays a central role in the pathogenesis of ischemic stroke. Increasing evidence suggests that it acts as a double-edged sword which exacerbates early brain injury, but also contributes to later repair. This protocol describes the isolation of immune cells from the ischemic brain and their subsequent flow cytometric phenotyping.
04:39
In Vitro Generation of Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors
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2025
This video demonstrates an in vitro assay to generate plasmacytoid dendritic cells (pDCs) from common lymphoid progenitor cells (CLPs). Upon co-culturing purified CLPs with γ-irradiated feeder cells in the presence of Fms-like tyrosine kinase-3 ligand (FL), the CLPs differentiate into non-adherent pDCs, the presence of which is confirmed using flow cytometry.
04:38
Analysis of Immune Cells in Mouse Nervous Tissues Using Flow Cytometry
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2025
Source: Hidmark, A. S., et al. Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry. J. Vis. Exp. (2017). This video demonstrates the use of flow cytometry to analyze immune cells in mouse sciatic nerves and dorsal root ganglia (DRGs). The process involves labeling immune cells with fluorophore- and biotin-tagged antibodies, stabilizing and permeabilizing the cells, and using fluorescent markers to detect cell-surface and nuclear...
04:09
Isolating Microglia from Mouse Brain Demyelinating Lesions Using Magnetic Activated Cell Sorting
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2025
This video demonstrates a protocol for extracting microglial cells from demyelinating lesions in mouse brains using magnetic cell sorting. The protocol involves dissecting the lesioned brain tissue containing microglial cells, treating the tissue with specific enzymes to obtain a single-cell suspension, and utilizing microglial receptor-specific magnetic beads to isolate the microglial cells.
FAQs
CD11b must pair with CD18 to form the Mac-1 integrin receptor. This pairing gives myeloid cells a molecular system for adhesion and migration, while also supporting complement recognition and phagocytosis. Consequently, CD11b measurements connect cell-surface phenotype with functional behaviors that help immune cells respond to tissue damage or infection.
Expression of CD11b can provide two related kinds of information: it helps identify myeloid populations and can indicate an activation state. In infection studies, changes in the CD11b-positive compartment can therefore be used to follow innate immune recruitment and to examine how inflammatory or immunosuppressive responses develop.
Mac-1 contributes to complement recognition and phagocytosis, so CD11b is relevant to how myeloid cells interact with targets during infection. Its role is broader than simple cell labeling: the same receptor context also supports adhesion and migration, linking recognition and uptake with movement through affected tissue.
Flow cytometry and immunostaining answer related but distinct experimental questions. Flow cytometry is used to detect and characterize CD11b-expressing cells, whereas immunostaining detects CD11b within the examined sample context. Selecting between them depends on whether the study prioritizes population characterization or detection of CD11b-associated responses in a specimen.
An analysis begins by detecting CD11b with flow cytometry or immunostaining, then relating the signal to the myeloid populations under study. Researchers can use the resulting measurements to characterize monocytes, macrophages, neutrophils, and other myeloid cells, while considering whether the goal is identity, activation, recruitment, or response assessment.
Within immunology and infection research, CD11b analysis helps connect innate immune-cell behavior with host–pathogen interactions. Researchers can track recruitment during infection, examine responses associated with inflammation or immunosuppression, and assess how myeloid populations are represented in a sample. These readouts provide cellular context for studying immune responses to infection and tissue damage.