Mouse Intestine
Mouse Intestine - Related Videos
06:55
Ileectomy-induced Bile Overaccumulation in Mouse Intestine
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Cited by 4 •
2017
Small intestine-dependent bile acid reabsorption and feedback inhibition of hepatic bile acid synthesis is important for systemic homeostasis and health. In this study, we describe a mouse model for ileal resection to evaluate ileectomy-induced bile malabsorption, overaccumulation, and toxicity in mouse intestine.
09:25
Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
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Cited by 68 •
2012
Here, we detail a methodology for the rapid isolation of mouse intestinal dendritic cells (DCs) and macrophages. Phenotypic characterization of intestinal DCs and macrophages is performed using multi-color flow cytometric analysis while magnetic bead enrichment followed by cell sorting is used to yield highly pure populations for functional studies.
05:49
A Simple Device to Rapidly Prepare Whole Mounts of the Mouse Intestine
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Cited by 10 •
2015
The use of a simple device to cut and ‘roll’ mouse intestines to rapidly prepare whole mount preparations is described.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
05:34
Oral Delivery of Transformed Yeast Cells into the Mouse Intestinal Immune System
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2025
This video showcases the oral administration of transformed yeast cells into a mouse model. The transformed yeast cells are orally administered using a gavage technique, reaching the mouse's stomach. Later, the cells migrate to the small intestine, where microfold cells engulf them and facilitate their transfer to Peyer's patches and gain access to the intestinal immune system.
FAQs
These defenses act as complementary layers rather than isolated components. The epithelial barrier limits direct access to underlying tissues, mucus helps regulate contact between intestinal contents and the surface, and antimicrobial factors constrain microbial exposure. Studying their combined activity helps explain how the intestine maintains host protection while remaining in contact with resident microbes and foreign antigens.
This distinction depends on coordinated innate and adaptive immune responses within the intestinal environment. Resident microbes can influence host defense without necessarily producing invasive disease, whereas pathogens may provoke stronger immune activity and tissue inflammation. Mouse intestinal models allow researchers to examine how immune cells respond to these different signals and how microbial exposure affects that balance.
Inflammation is both an immune response and a potential source of tissue disruption. In the mouse intestine, inflammatory activity can alter barrier function, changing how microbes and foreign antigens interact with host tissues. Examining this relationship helps researchers connect immune responses with disease processes such as intestinal infection and inflammatory bowel disease.
Researchers use these models to examine how intestinal innate and adaptive immune cells respond to microbes encountered in the gut. The approach connects microbial exposure with changes in barrier function, inflammation, and host defense. It is therefore useful for studying both protective responses against enteric pathogens and immune dysregulation associated with intestinal disease.
Mouse intestinal studies can clarify how enteric pathogens interact with host barriers and immune defenses during infection. They may also help investigators relate microbial exposure to transmission-related processes and inflammatory outcomes. This makes the model relevant for understanding pathogen spread through the intestine while evaluating how host responses influence infection severity and persistence.
The intestinal model provides a setting for examining how immune responses develop in relation to enteric microbes and foreign antigens. Researchers can use it to investigate vaccine responses and to assess therapeutic concepts aimed at intestinal infection. Findings may also reveal how microbiota-associated host defense and inflammation influence the success of these interventions.