Nanoparticle Labeling
Nanoparticle Labeling - Related Videos
08:26
Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
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Cited by 20 •
2015
Targeted cell delivery is useful in a variety of biomedical applications. The goal of this protocol is to use superparamagnetic iron oxide nanoparticles (SPION) to label cells and thereby enable magnetic cell targeting approaches for a high degree of control over cell delivery and localization.
05:05
Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle
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Cited by 95 •
2011
We describe a technique for labeling and tracking stem cells with FDA-approved, superparamagnetic iron oxide (SPIO), ferumoxytol (Feraheme). This cellular imaging technique that utilizes magnetic resonance (MR) imaging for visualization, is readily accessible for long-term monitoring and diagnosis of successful or unsuccessful stem cell engraftments in patients.
07:23
An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
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Cited by 5 •
2016
A detailed procedure for the synthesis of a 125I-labeled azide and the radiolabeling of dibenzocyclooctyne (DBCO)-group-conjugated, 13-nm-sized gold nanoparticles using a copper-free click reaction is described.
08:14
Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
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Cited by 1 •
2015
This manuscript describes a novel technique which allows the detection of intracellular gene expression after endocytosis of fluorescence-labeled nanoparticles directly in live cells. The method does not require manipulation of the cells and is not restricted with respect to the target gene or species.
09:23
Harmonic Nanoparticles for Regenerative Research
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Cited by 1 •
2014
Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also presented.
FAQs
These approaches place detectable tags in different locations and through different interactions. Surface adsorption holds a label on the particle exterior, encapsulation incorporates it within the particle, and covalent coupling attaches it through a chemical bond. Selecting among them requires maintaining the particle’s size, composition, and biological function so measurements reflect nanoparticle behavior rather than labeling-related changes.
A label can affect how a nanoparticle is recognized, distributed, taken up by cells, or delivered to a target. Preserving the original particle properties helps researchers interpret detected signals as evidence of the nanoparticle’s biological behavior. This is especially important when studying immune recognition or evaluating whether a therapeutic or diagnostic design performs as intended.
Detectable tags allow researchers to visualize whether labeled particles interact with immune cells and to monitor cellular uptake. Tracking these events connects nanoparticle design with immune recognition and biological distribution. In immunology studies, the resulting observations can help explain how a particle reaches cells and whether its design supports vaccine, diagnostic, or delivery objectives.
Researchers should consider the label type, where the tag will be placed, and whether the introduction method preserves the particle’s relevant properties. Fluorescent dyes, isotopes, and affinity molecules provide different ways to detect or follow particles. The choice should match the intended measurement, such as visualization, tracking, characterization, or assessment of delivery to a biological target.
A general workflow begins by selecting a detectable tag suited to the intended measurement, followed by introducing it through surface adsorption, encapsulation, or covalent coupling. Researchers then use the labeled particles to identify, track, or characterize their behavior in biological systems. The particle’s size, composition, and biological function remain important reference points throughout interpretation.
In infection research, labeling can show tissue distribution, cellular uptake, and delivery toward pathogens or infected sites. These observations help assess whether a nanoparticle reaches the intended biological location and whether its design supports antimicrobial delivery or diagnostic probing. The same tracking information can also contribute to imaging tools focused on infection-related processes.
Labeled particles can provide evidence about where nanoparticles travel, which cells take them up, how they interact with immune systems, and whether they reach pathogens or infected tissues. These outcomes connect particle design with immune recognition and therapeutic performance. Consequently, labeling supports development and evaluation of vaccines, diagnostic probes, antimicrobial delivery systems, and imaging tools.