Circulating Rna
Circulating Rna - Related Videos
04:33
Circulating miRNA Extraction: A Method to Isolate miRNA from Plasma Samples Via Organic Extraction and Small RNA Enrichment
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2023
This video describes the extraction of circulating microRNA from plasma samples of colorectal cancer patients using organic extraction followed by glass-fiber column enrichment. miRNAs are stable in biological fluids such as serum and plasma, which renders them ideal circulating biomarkers for cancer diagnosis, prognosis, and treatment decision-making and monitoring. This method will help identify new circulating biomarkers capable of predicting clinical outcomes in patients.
01:14
Fetal Circulation
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2024
Fetal circulation is a unique system that facilitates the exchange of gases, nutrients, and waste products between the developing fetus and the mother. This intricate process takes place through a special organ called the placenta. Two umbilical arteries transport blood from the fetus to the placenta. At the placenta, the blood absorbs oxygen and nutrients while simultaneously eliminating waste products. This oxygen-enriched and nutrient-rich blood then returns to the fetus through one...
01:21
Coronary Circulation
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2024
The heart, an organ critical to survival, gets nourishment not from the blood it pumps but from a separate circulation system known as coronary circulation. This is the shortest circulation in the body and is responsible for supplying the heart with the nutrients it needs to function effectively. Coronary circulation begins at the base of the aorta, where two main arteries arise—the left and right coronary arteries. These arteries encircle the heart in the coronary sulcus and supply the...
08:10
Capture and Release of Viable Circulating Tumor Cells from Blood
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Cited by 2 •
2016
A protocol to utilize a poly(N-iso-propylacrylamide) (PIPAAm) coated microfilter for effective capture and thermoresponsive release of viable circulating tumor cells (CTC) is presented. This method allows capture of CTC from patients' blood and subsequent release of viable CTC for downstream off-chip culture, analyses and characterization.
09:54
RNA-Seq
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2023
Among different methods to evaluate gene expression, the high-throughput sequencing of RNA, or RNA-seq. is particularly attractive, as it can be performed and analyzed without relying on prior available genomic information. During RNA-seq, RNA isolated from samples of interest is used to generate a DNA library, which is then amplified and sequenced. Ultimately, RNA-seq can determine which genes are expressed, the levels of their expression, and the presence of any previously unknown transcripts.
FAQs
Circulating RNA reaches body fluids through several biologically distinct routes. Cells can actively secrete RNA, package it into extracellular vesicles, or release it when they undergo cell death. These routes affect how the signal enters circulation and may influence how well it reflects tissue activity or disease-associated changes.
Extracellular vesicles and proteins help shield RNA from rapid degradation in body fluids. Vesicle packaging physically encloses RNA, while protein association provides another stabilizing context. This protection matters because intact or sufficiently preserved molecules are more likely to remain measurable, improving the chance that detected patterns can be related to tissue or disease states.
Analysis can focus on different RNA classes, including messenger RNA, microRNA, and other RNA species. It may assess either their abundance or their sequence, so the resulting information is not limited to how much RNA is present. Choosing the RNA class and measurement type determines which molecular changes can be investigated in a medical sample.
Reliable interpretation starts before measurement. Blood or other body-fluid samples require careful handling because RNA stability can affect the signal that is ultimately observed. Analytical standardization is also important: without consistent approaches to processing and measurement, differences between samples may reflect technical variation rather than biology, complicating comparisons and clinical translation.
In medicine, circulating RNA measurements may support investigation of cancer, cardiovascular disease, and infection. They can also contribute to evaluating treatment response. Because the same general approach can be applied across disease settings, researchers can examine whether disease-associated molecular patterns change over time or differ among patient groups, while using minimally invasive sampling.
Measured abundance or sequence can provide information for disease detection, patient stratification, and treatment monitoring. These uses address different questions: identifying a disease-associated signal, distinguishing patients according to molecular patterns, or following change during therapy. The approach still faces challenges involving sample handling, RNA stability, and analytical standardization before broad clinical use.