In Vivo Labeling
In Vivo Labeling - Related Videos
12:32
Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
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Cited by 14 •
2013
Retrograde transport of fluorescent dye labels a sub-population of neurons based on anatomical projection. Labeled axons can be visually targeted in vivo, permitting extracellular recording from identified axons. This technique facilitates recording when neurons cannot be labeled through genetic manipulation or are difficult to isolate using 'blind' in vivo approaches.
09:49
Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
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Cited by 39 •
2010
Many infections elicit a strong CTL response, but occasionally, the quantity of responding cells does not correlate to control of the pathogen1. One measure of CTL quality is their ability to kill specifically2. CFSE labeling of target cells can be used to investigate this CTL response quality in vivo3,4.
09:06
Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
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Cited by 18 •
2008
For the evaluation of new stem cell therapies it is important to non-invasively track the injected cells in vivo. This video will show you how to label human mesenchymal and embryonic stem cells with iron oxide based contrast agents in vivo for subsequent MR imaging in vivo.
09:21
Viral-mediated Labeling and Transplantation of Medial Ganglionic Eminence (MGE) Cells for In Vivo Studies
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Cited by 35 •
2015
GABAergic cortical interneuron progenitors disperse, develop and synaptically integrate into a host cortex after transplantation. These cells can be easily transduced before transplantation for in vivo studies of genetically modified GABAergic precursors. Here, we show viral labeling techniques to target specific interneuron subgroups using existing Cre lines and Cre-dependent reporters.
09:19
Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
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Cited by 3 •
2025
This protocol presents a step-by-step guide for the Simultaneous Label-free Autofluorescence Multi-harmonic (SLAM) microscopic technique, including details on how to generate the laser light source, prepare a tissue sample, conduct imaging, and analyze the data. SLAM advances nonlinear microscopy by measuring four complementary label-free contrasts to investigate the tissue microenvironment.
FAQs
The information obtained depends on how the label relates to its target. Genetic reporters identify cells through an introduced biological signal, fluorescent probes provide detectable molecular marks, antibodies bind selected targets, and tracers reveal transport along neural pathways. Choosing among these approaches links the observed signal to cellular identity, molecular location, or anatomical connectivity.
Specificity determines whether a detected signal can be interpreted as evidence about a particular cell, molecule, or structure. Antibodies and probes can associate signals with selected targets, while genetic reporters can distinguish labeled cell populations. This relationship helps researchers connect molecular identity with neural anatomy rather than treating every detected signal as equivalent.
Some tracers are transported along neural pathways, so their distribution can provide information about anatomical connections. The resulting pattern can be examined with imaging or histological methods and compared across regions. This makes transport-based labeling especially relevant when the research question concerns pathway organization rather than only the presence of an individual molecular target.
A typical workflow begins by introducing a suitable genetic reporter, fluorescent probe, antibody, or tracer into the living organism. Researchers then detect the label with imaging or histological methods and analyze its location or pattern. Repeated observations can show how labeled cells, molecules, or structures change over time while biological context is retained.
The choice follows the information the experiment must obtain. Genetic reporters may support identification of cells, probes or antibodies can associate signals with molecular targets, and tracers can address neural pathways. If the goal includes monitoring activity, migration, development, disease-related change, or treatment effects, the method should produce a detectable signal suited to that outcome.
In neuroscience, the approach can support studies of neuronal connections, cell migration, and changes in neural circuit organization or activity. Researchers can apply it during development or when examining disease and treatment-related changes. Its main value is allowing molecular identity to be interpreted alongside dynamic brain structure and function within the living system.