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Problems in the processing of biological samples for scanning electron microscopy observation include cell collapse, treatment of samples from wet microenvironments and cell destruction. Low-cost and relatively rapid protocols suited for preparing challenging samples such as floral meristems, oomycete cysts, and fungi (Agaricales) are compiled and detailed here.
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Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples
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Bello, M. A., Ruiz-León, Y., Sandoval-Sierra, J. V., Rezinciuc, S., Diéguez-Uribeondo, J. Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples. J. Vis. Exp. (120), e55031, doi:10.3791/55031 (2017).
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