ELISA Methods for Detecting Antigens

ELISA methods detect antigens, antibodies, proteins, hormones, peptides, and other biomolecules in biological samples. Enzyme-linked immunosorbent assay is highly sensitive because it can detect very low antigen levels. It works by using an antigen-antibody interaction, then an enzyme-linked antibody turns a colorless substr...

Enzyme-linked Immunosorbent Assay, or ELISA is a highly sensitive quantitative assay commonly used to measure the concentration of an analyte like cytokines and antibodies in a biological sample. The general principle of this assay involves three steps: starting with capture, or immobilization, of the target analyte on a micro plate, followed by...