Protein Pull-Down with Antibodies and Beads

Immunoprecipitation uses antibodies and Protein A/G agarose beads to isolate a specific protein from a cell lysate. It is also called a pull-down assay. The term "immuno" refers to the antibody, and "precipitation" means pulling a substance out of solution.

The target protein ...

Immunoprecipitation, or IP, is a widely used technique to isolate a protein of interest from a cell or tissue lysate or a body fluid for protein characterization or to investigate protein-protein interactions.

The process begins with an antibody, which has a high affinity and specificity for the target protein. This antibody is mixed with ...