ELISPOT Assay for Single-Cell Immune Detection

ELISPOT is a sensitive assay for detecting immune cells that release a specific protein. It is based on an ELISA, or enzyme-linked immunosorbent assay, but it shows single-cell responses as visible spots. The method was first described in 1983 by Czerkinsky to count B cell hybridomas that produce antigen-specific immunoglobu...

The Enzyme-linked Immunospot, or ELISPOT, assay is a method to analyze the immune response to a pathogen or cell damage. It allows for quantification of the activation of different immune cells by detecting specific proteins they secrete. For example, ELISPOT is commonly used for measuring T-cell responses upon exposure to a foreign antigen by d...