Guest Editor
Collection Overview
Extracellular vesicles (EV) are emerging as new mediators of cell-to-cell communication, involved in the pathophysiology of a plethora of diseases. EV are a composite of heterogeneous phospholipid bilayer-surrounded particles ranged between 30 and 5000nm in diameter, namely exosomes, microvesicles and apoptotic bodies. EV are potentially generated and released by almost all eukaryotic cells, and contain bioactive compounds (such as lipids, proteins, RNA and metabolites), determining its mechanism of action and also reflecting the state of its cell of origin. Given their pathophysiological relevance, EV have been the object of intensive research in the last decade. However, research in the properties, structure, composition, pathophysiological activity and metabolism/clearance of EV is still technically limited by the EV extraction and purification methodology. This method collection is aimed at reporting methods of isolation and purification of EV, for further downstream applications.
Editorial
Articles
Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
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Cited by 7
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2022
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Yuan Cao*1,2, Ji-Yu Qiu*1,3, Da Chen1,2, Chen-Yao Li1,4, Shu-Juan Xing1,5, Chen-Xi Zheng1, Xin Liu2, Yan Jin1,6, Bing-Dong Sui1
1State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, 2Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 3Department of VIP Dental Care, School of Stomatology, The Fourth Military Medical University, 4School of Basic Medicine, The Fourth Military Medical University, 5College of Life Science, Northwest University, 6Xi'an Institute of Tissue Engineering and Regenerative Medicine
Enrichment of Astrocyte-Derived Extracellular Vesicles from Human Plasma
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Cited by 16
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2022
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Natalia Valle-Tamayo1,2, Rocío Pérez-González2,3, Gemma Chiva-Blanch4,5, Olivia Belbin1,2, Sara Serrano-Requena1,2, Sònia Sirisi1,2, Alba Cervantes González1,2, Oriol Giró4,5, Érika Sánchez-Aced1,2, Oriol Dols-Icardo1,2, Daniel Alcolea1,2, María Carmona-Iragui1,2, Amanda Jimenez4,5, Alberto Lleó1,2, Juan Fortea1,2, M. Florencia Iulita1,2
1Sant Pau Memory Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau, Universitat Autònoma de Barcelona, 2Center of Biomedical Investigation Network for Neurodegenerative Diseases (CIBERNED), 3Movement Disorders Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, 4Department of Endocrinology and Nutrition, Hospital Clínic de Barcelona, August Pi i Sunyer Biomedical Research Institute - IDIBAPS, 5Spanish Biomedical Research Network in Physiopathology of Obesity and Nutrition (CIBEROBN)
A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells
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Cited by 8
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2022
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Benson Koh1,2, Kian Leong Tan3, Hong Hao Chan4, Qi Hao Daniel Looi2, Moon Nian Lim5, Chee Wun How4, Jia Xian Law1, Jhi Biau Foo6,7
1Centre for Tissue Engineering and Regenerative Medicine, Universiti Kebangsaan Malaysia Medical Centre, 2My CytoHealth Sdn. Bhd., 3School of Biosciences, Faculty of Health and Medical Sciences, Taylor's University, 4School of Pharmacy, Monash University Malaysia, 5Haematology Unit, Cancer Research Centre, Institute for Medical Research (IMR), National Institutes of Health (NIH), Ministry of Health Malaysia, 6School of Pharmacy, Faculty of Health & Medical Sciences, Taylor's University, 7Centre for Drug Discovery and Molecular Pharmacology (CDDMP), Faculty of Health and Medical Sciences, Taylor's University
Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells
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Cited by 5
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2022
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Shu-Juan Xing1,2, Kai-Chao Zhang2,3, Si-Yuan Tang2,4, Lu Liu2,5, Yuan Cao2,5, Chen-Xi Zheng2, Bing-Dong Sui2, Yan Jin2,3
1College of Life Science, Northwest University, 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, 3Xi'an Institute of Tissue Engineering and Regenerative Medicine, 4School of Basic Medicine, Fourth Military Medical University, 5Department of Orthodontics, School of Stomatology, Fourth Military Medical University
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
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Cited by 3
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2023
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Abstracts
Amniotic Fluid-derived Exosomes Isolation Protocol
Halimeh Mobarak1,
Mahdi Mahdipour*1,
Reza Rahbarghazi*2
1Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran,
2Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
Isolation and beads-based analysis of cytokines associated with extracellular vesicles
Anna Lebedeva*1,
Elena Vasilieva1,
Leonid Margolis2,
Daria Vorobyeva1,
Wendy Fitzgerald2,
Alexander Shpektor1,
Elena Maryukhnich1
1Laboratory of Atherothrombosis, Moscow State University of Medicine and Dentistry,
2Section on Intercellular Interactions, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health
Optimized methods for isolating extracellular vesicles from biofluids with Size Exclusion Chromatography
Dmitry Ter-Ovanesyan*1
1Harvard University