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Current Methods for Imaging and Quantifying Proteins, Nucleic Acids, and Metabolites in the Tumor Microenvironment (TME)

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Guest Editors

Abhishek Thavamani

Abhishek Thavamani

Harvard Medical School and Beth Israel Deaconess Medical Center

Dr. Thavamani completed his PhD research through the International Max Planck Research School, at the University of Tubingen, Germany. His research focused on studying specific subtypes of liver carcinoma using mouse models, with a goal of developing novel treatment strategies. Since 2019, he has been pursuing his research as a post-doctoral fellow at the Beth Israel Deaconess Medical center and Harvard Medical School. His research currently focuses on developing/improving targeted therapy strategies for the treatment of triple negative breast cancer (TNBC). His research interests include, studying the heterogeneity of tumors and the surrounding microenvironment in vivo using novel-imaging technologies.

Maulik Vyas

Maulik Vyas

Harvard Medical School and Massachusetts General Hospital

Dr. Maulik Vyas earned PhD in 2016 from the University of Cologne in Germany, where he developed novel bi- and tri-specific antibody-based recombinant constructs to harness Natural Killer (NK) cells against leukemia. He continued in the same lab as a postdoctoral scientist studying immune suppression by soluble factors within the ascites of ovarian cancer patients. In 2019, he joined the Cutaneous Biology Research Center at Massachusetts General Hospital and Harvard Medical School to study the regulation of NK cell effector functions in solid cancers. Using skin and breast cancer models, his efforts have been to identify novel components in the tumor microenvironment that modulate NK cell functions and to translate these findings to facilitate the development of immunotherapies against cancer.

Lin Wang

Lin Wang

Harvard Medical School and Beth Israel Deaconess Medical Center

Dr. Wang received his PhD degree from the University of Science and Technology of China in 2018, and his research focused on cancer metabolism and cell cycle regulation during this period. He joined BIDMC in 2018 and continued the research on targeting cancer metabolism for combinational therapy with immune components modulation. He is experienced in using different ways to detect metabolic changes in cancer cells.

Steven Vandal

Steven Vandal

Harvard Medical School and Beth Israel Deaconess Medical Center

Dr. Vandal received his PhD in Physics in 2020 from the Worcester Polytechnic Institute in Massachusetts. As part of his graduate studies, he worked on simulations of cytoskeletal cargo transport to understand the bio-mechanical properties of molecular motors. In 2021, he joined Beth Israel Deaconess Medical Center in Boston, as a post-doctoral fellow. He currently studies the cellular metabolism of breast cancer in relation to the cytoskeletal network.

Collection Overview

In recent years, there has been a paradigm shift in cancer research. In addition to studying the tumors, the tumor microenvironment (TME) also has been widely studied. The different components of TME play a critical role in modulating important tumor milestones such as initiation, progression, metastasis and response to various therapies. 

Proteins: A milieu of proteins in TME including the Extracellular matrix (ECM) perform numerous functions that range from providing structural scaffolding to modulating the growth and movement of tumor and stromal cells. Technological improvements in protein purification, proteomics, imaging techniques and computational tools have extended our understanding of the complex interplay between the ECM and surrounding cells at different stages of cancer progression. 

Nucleic Acids: Unique nucleic acid signatures in the TME have revealed regulatory mechanisms associated with tumor development and therapy resistance. Nucleic acid changes have also been used as prognostic factors for various therapies. Advancements in sequencing technologies coupled with increased computational capabilities have resulted in novel methodologies to investigate nucleic acid signatures. 

Metabolites: Detection of metabolites in cells or tissues is critical to understanding the metabolic processes in different physiological conditions. Novel technologies such as in situ mass spectrometry and Magnetic Resonance Imaging (MRI) have made it possible to detect and visualize metabolites throughout the tissue in a spatial and temporal manner.

This collection will include: 

  1. Methodologies to investigate the above components of TME individually or simultaneously. 
  2. Novel computational tools or packages associated with the analysis, visualization and quantification of the different components of the TME.

Articles

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment
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Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment

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Cited by 7

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2023

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