Mammalian CRISPR Genome Editing

TOPICAL COLLECTIONS

Guest Editor

Nozomu Yachie

The University of British Columbia, School of Biomedical Engineering, Vancouver, Canada

Dr. Yachie is an Associate Professor and Canada Research Chair Tier 2 in Synthetic Biology at the School of Biomedical Engineering (SBME) at the University of British Columbia (UBC). His research program (https://yachie-lab.org/) is combining technologies in cell engineering, genome editing, and computational biology and developing new measurement technologies for mammalian development and cell differentiation. Dr. Yachie received his PhD degree in Systems Biology from Keio University in 2009 and completed his postdoctoral training at Dr. Fritz Roth’s lab from Harvard Medical School and the University of Toronto. He is also a recipient of the JSPS DC1 (2007-2009), PD (2009-2010), and “Research Abroad” (2010-2012) fellowships from the Japan Society for the Promotion of Science, the Banting Postdoctoral Fellowship (2012-2014), the PRESTO Researcher-ship awarded by the Japan Science and Technology Agency (2014-2018), and the Young Scientists Award by the Minister of Education and Science, Japan (2020).

Collection Overview

CRISPR–Cas9 genome editing has sparked a revolution in biology and enhanced our capability of genetically engineering mammalian cells and animal models. High-throughput genetics and genome engineering are no longer only for the biology of microorganisms. New screening, precise gene editing, epigenetic modification, and synthetic biology methods founded on CRISPR have kept emerging and unveiled molecular and cellular mechanisms in mammalian systems. CRISPR’s simplicity, modularity, and powerfulness of activating its function in condition with a target DNA code continue to stimulate researchers’ creativity to develop new ways of interrogating biological systems. The pace of technology and experimental method development with CRISPR is unstoppable. To further advance mammalian biology even faster with the community, this collection aims to provide video protocols on cutting-edge CRISPR technologies developed to study mammalian cells and animals.

Articles

8:48

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

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Cited by 13

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2022

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Yoko Tanimoto*¹, Natsuki Mikami*^1,2, Miyuki Ishida¹, Natsumi Iki¹, Kanako Kato¹, Fumihiro Sugiyama¹, Satoru Takahashi¹, Seiya Mizuno¹

¹Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba, ²Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba

6:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

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Cited by 2

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2022

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Kyle E. Simpson¹, Shoaib Faizi¹, Ravichandra Venkateshappa¹, Mandy Yip¹, Raj Johal¹, Damon Poburko¹, Yen May Cheng¹, Diana Hunter¹, Eric Lin¹, Glen F. Tibbits¹, Thomas W. Claydon¹

¹Department of Biomedical Physiology and Kinesiology, Simon Fraser University

10:57

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

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2022

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Manabu Ozawa*¹, Chihiro Emori*², Masahito Ikawa^1,2

¹Laboratory of Reproductive Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, ²Research Institute for Microbial Diseases, Osaka University

Mouse <em>In Vivo</em> Placental Targeted CRISPR Manipulation

7:39

Mouse In Vivo Placental Targeted CRISPR Manipulation

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Cited by 5

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2023

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Annemarie J. Carver^1,2,3, Robert J. Taylor^2,3, Hanna E. Stevens^1,2,3,4

¹Interdisciplinary Graduate Program in Genetics, University of Iowa, ²Department of Psychiatry, Carver College of Medicine, University of Iowa, ³Iowa Neuroscience Institute, Carver College of Medicine, University of Iowa, ⁴Hawk-IDDRC, University of Iowa

7:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

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Cited by 11

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2023

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Shaohui Zheng^1,2, Fang Liang³, Yu Zhang^1,2, Ji-Feng Fei⁴, Wei Qin^1,2, Yanmei Liu^1,2

¹Key Laboratory of Brain, Cognition and Education Science, Ministry of Education, ²Institute for Brain Research and Rehabilitation, Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University, ³Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Engineering Technology Research Center for Environmentally Friendly Aquaculture, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Sciences, South China Normal University, ⁴Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences

Somatic Genome-Engineered Mouse Models Using <em>In Vivo</em> Microinjection and Electroporation

8:06

Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation

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Cited by 1

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2023

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Keerthana Harwalkar^1,2, Nobuko Yamanaka^1,3, Yojiro Yamanaka^1,2,3

¹Rosalind and Morris Goodman Cancer Research Institute, McGill University, ²Department of Human Genetics, McGill University, ³McGill Integrated Core for Animal Modeling (MICAM), McGill University

Abstracts

Method of generating floxed mice by combining in vitro fertilization with electroporation and CRISPR-Cas9 genome editing.

Hirofumi Nishizono^*1

¹Medical Research Institute, Kanazawa Medical University 1‑1 Daigaku, Uchinada, Ishikawa 920‑0293, Japan

Fluorescence-based reporters for CRISPR base editors

Arman Adel¹,

Nozomu Yachie^*2

¹The School of Biomedical Engineering, the University of British Columbia,

²The School of Biomedical Engineering, the University of British Columbia; Research Center for Advanced Science and Technology, the University of Tokyo