Guest Editors
Gavin McStay
Liverpool John Moores University, Senior Lecturer
Dr Gavin McStay is a Senior Lecturer at Liverpool John Moores University in the United Kingdom. He completed his PhD at the University of Bristol under the supervision of Professor Andrew Halestrap and his aim was to characterize the mitochondrial permeability transition pore. He carried out his post-doctoral work with Douglas Green at St Jude Children’s Research Hospital on cell death and Alexander Tzagoloff at Columbia University on cytochrome c oxidase assembly. His current research interests focus on how mitochondrial functions are regulated.
Mário H Barros
University of Sao Paulo-Brazil, Department of Microbiology
Dr. Mário H Barros is an Associate Professor at the Department of Microbiology- University of Sao Paulo-Brazil. He graduated in Biological Sciences (1993) and holds a PhD in Genetic Sciences (2000) from the same university. He trained as a postdoc in Alexander Tzagoloff’s laboratory at Columbia University. His study focuses on proteins of unknown function that are required for mitochondria biogenesis in the yeast Saccharomyces cerevisiae.
Collection Overview
Mitochondria represents the most significant production site of ATP in eukaryotic cells, ranging from unicellular fungi to complex multicellular metazoans. Mitochondrial ATP production is a product of gene expression from both the nuclear and mitochondrial genomes in the context of a double membrane organelle. Evaluation of gene expression and function is essential to understand how mitochondrial biogenesis is controlled to ensure the provision of efficient ATP production. Mitochondria are the hub of energy metabolism. In addition to ATP synthesis, the organelle has different functions in bioenergetic processes such as redox metabolism and signaling. This has resulted in the development and use of techniques ranging from genetic, biochemical, structural, molecular, and cellular biology approaches. This Methods Collection will highlight the diversity of techniques that assess gene expression control, protein maturation, protein complex assembly and structure, redox homeostasis, along with mitochondrial function and morphology from model systems to clinical samples.
Articles
Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer
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Cited by 3
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2021
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1Department of Pathology, University of Michigan, Ann Arbor, 2Department of Internal Medicine, University of Michigan, Ann Arbor, 3Department of Cellular and Developmental Biology, University of Michigan, Ann Arbor, 4Rogel Cancer Center, University of Michigan, Ann Arbor, 5Institute of Gerontology, University of Michigan, Ann Arbor
Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes
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2021
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Daniel Barrera1, Sierra Upton2, Megan Rauch3, Tara Notarianni4, Ki Suk Eum5, Megan Liberty6, Sarmila Venkoba Sah7, Robert Liu8, Sean Newcomer9, Linda E. May10, Emre Agbas11, Jessica Sage12, Edina Kosa7, Abdulbaki Agbas7,13
1AdventHelath, 2University of Illinois Chicago School of Public Health, 3Lincoln Memorial University, 4Children’s Mercy Hospital, 5Scripps Clinic, 6Cincinnati Children’s Hospital Medical Center, 7Kansas City University, 8Roblex Rex Veterans Affairs Medical Center, 9California State University San Marcos, 10East Carolina University, 11Massachusetts Institute of Technology, 12Boehringer Ingelheim Norway KS, 13Heartland Center for Mitochondrial Medicine
Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates
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Cited by 4
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2021
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1Integrated Physiology and Molecular Medicine Laboratory, Pennington Biomedical Research Center, 2Clinical Oncology and Metabolism, Pennington Biomedical Research Center, 3Department of Nutrition, Case Western Reserve University, 4Department of Clinical Nutrition, University of Texas Southwestern Medical Center
Single-plate Runs of Mito Stress And Glycolysis Stress Tests using Adherent or Non-Adherent Cells
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2026
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Jane Tonello1, Salvador Gonzalez Ochoa1, Thanigaivelan Kanagasabai1,2, Muna Mohammed2, Anil Shanker1,3,4, Alla V. Ivanova1,2
1Department of Biochemistry, Cancer Biology, Neuroscience and Pharmacology, School of Medicine, Meharry Medical College, 2Department of Biomedical Sciences, School of Graduate Studies, Meharry Medical College, 3Host-Tumor Interactions Research Program, Vanderbilt-Ingram Comprehensive Cancer Center, Vanderbilt University School of Medicine, 4Vanderbilt Institute for Infection, Immunology and Inflammation, Vanderbilt University School of Medicine
Abstracts
Measuring Dynamic Changes in Mitochondrial Membrane Potential and Trafficking in Sensory Neurites
Amy E. Rumora1,
Eva L. Feldman1,
Stephen I. Lentz*2
1Department of Neurology, University of Michigan, Ann Arbor, MI,
2Department of Internal Medicine, Division of Metabolism, Endocrinology & Diabetes, University of Michigan, Ann Arbor, MI
Stop-flow respirometry in Drosophila as a proxy for mitochondrial function.
Richard Sejour1,
Heather Liwanag2,
Roger A. Sanguino1,
Eugenia Villa-Cuesta*1
1Adelphi University,
2California Polytechnic State University
Optimized Seahorse protocols for single-plate runs of Mito Stress and Glycolysis Stress tests using mouse BMDMs and T cells
alla Ivanova*1
1Meharry Medical School, Nashville, TN
Deciphering the foundation of acquired mitochondrial dysfunction involving magnesium, selenium, iron and coenzyme Q10 deficiencies
Roy Moncayo*1,
Helga Moncayo1
1WOMED, Karl-Kapferer-Strasse 5, Innsbruck, Austria