Automated Infrared Pupillometer

An automated infrared pupillometer is a noninvasive instrument that measures pupil size and reactivity to characterize visual and autonomic behavior. It uses infrared illumination and a camera to detect the pupil under controlled lighting, then software tracks changes in diameter and calculates responses to light or other stimuli. These measurements provide objective indicators of arousal, attention, sensory processing, and autonomic nervous system activity without relying on subjective observation. In behavioral research, automated pupillometry supports precise comparisons across individuals and experimental conditions, while its standardized recording and analysis can improve the study of cognition, emotion, perception, and neurological function.

Automated Infrared Pupillometer - Related Videos

Education

JoVE Science Education - Chemistry
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Infrared Spectroscopy

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2023

Source: Vy M. Dong and Zhiwei Chen, Department of Chemistry, University of California, Irvine, CA This experiment will demonstrate the use of infrared (IR) spectroscopy (also known as vibrational spectroscopy) to elucidate the identity of an unknown compound by identifying the functional group(s) present. IR spectra will be obtained on an IR spectrometer using the attenuated total reflection (ATR) sampling technique with a neat sample of the unknown.

Research

JoVE Journal - Engineering

Characterizing Far-infrared Laser Emissions and the Measurement of Their Frequencies

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Cited by 4 •

2015

We describe the generation of far-infrared radiation using an optically pumped molecular laser along with the measurement of their frequencies with heterodyne techniques. The experimental system and techniques are demonstrated using difluoromethane (CH2F2) as the laser medium whose results include three new laser emissions and eight measured laser frequencies.

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

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Cited by 1 •

2016

Peptide adsorption to plasticware during traditional tip-based serial dilutions can significantly impact potency determination and confound the understanding of structure-activity relationships used for lead identification and lead optimization phases of drug discovery. Here methods for automated acoustic non-contact serial dilution of peptide samples are described.

Near-infrared Fluorescence Imaging of Abdominal Aortic Aneurysms

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2023

Source: Arvin H. Soepriatna1, Kelsey A. Bullens2, and Craig J. Goergen1 1 Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 2 Department of Biochemistry, Purdue University, West Lafayette, Indiana Near-infrared fluorescence (NIRF) imaging is an exciting optical technique that utilizes fluorescent probes to visualize complex biomolecular assemblies in tissues. NIRF imaging has many advantages over conventional imaging methods for noninvasive imaging of diseases.

Performing Electroconvulsive Therapy and Assessing Seizure Efficacy via Pupillary Response

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2025

Source: Shirozu, K., et al. Pupillary Response as Assessment of Effective Seizure Induction by Electroconvulsive Therapy. J. Vis. Exp. (2019)This video demonstrates the procedure for performing electroconvulsive therapy in a human patient, highlighting the stimulation process and assessing seizure activity through pupillary response.

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