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Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
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Genetics
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JoVE Journal Genetics
Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

DOI:
10.3791/55369-v

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07:41 min

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October 02, 2017

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, ,
1Department of Medicinal Chemistry and Molecular Pharmacology,Purdue University

Chapters

  • 00:05Title
  • 00:55Basic Sequential Salt Extraction (SSE)
  • 05:10Results: Evaluation of Elution Patterns with Sequential Salt Extraction
  • 07:03Conclusion

Summary

Automatic Translation

Automatic Translation

Sequential salt extraction of chromatin bound proteins is a useful tool for determining the binding properties of large protein complexes. This method can be employed to evaluate the role of individual subunits or domains in the overall affinity of a protein complex to bulk chromatin.

Tags

Keywords: Chromatin BindingSequential Salt ExtractionChromatin Modifying ComplexesChromatin ImmunoprecipitationEpigenetic RegulatorsProtein Binding ProfilesSalt-free Modified Ripa BufferSodium Chloride

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